Dictionary » E » Endonuclease

Endonuclease

Definition

noun, plural: endonucleases

A nuclease that cleaves the phosphodiester bond between nucleotides situated within the polynucleotide chain, hence, producing fragments of various sizes.


Supplement

A common example of endonuclease is restriction endonuclease.


Word origin: Greek éndon (within) + nuclease.

Compare: exonuclease

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Souther blot and restriction mapping

... help with constructing a restriction map from Southern Hybridisation data. I can construct restriction maps from fragment data obtained through endonuclease digestion and separation by standard gel electrophoresis, however, Southern Blot data only shows those fragments that a probe has hybridised ...

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by Mistersnuffle
Sat Oct 04, 2014 11:17 am
 
Forum: Genetics
Topic: Souther blot and restriction mapping
Replies: 0
Views: 18

Restriction Digesion

which is the best option for Restriction Endonuclease digesion ??? A) 37' C water bath B) 37' C PCR program

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by Helics
Sun Apr 06, 2014 4:07 pm
 
Forum: Molecular Biology
Topic: Restriction Digesion
Replies: 3
Views: 929

Find number of restriction fragment size in chromosome

Hi all, I am prepare in entrance exam. I need help. A restriction endonuclease has the recognition sequence G/AATTC, where "/" indicate the cut side. This sequence is found, on average, once every 'X' residues in a chromosome. 'X' = Options a. 146 ...

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by kanagasundar
Wed Nov 21, 2012 1:38 am
 
Forum: Molecular Biology
Topic: Find number of restriction fragment size in chromosome
Replies: 1
Views: 1108

Recombinant DNA not degraded inside transformed cells, why?

When a foreign DNA enters a bacterial cell, there is a race between methylases and restriction endonucleases. If the DNA is methylated as "self" before the corresponding endonuclease arrives, the DNA will not be sliced. I'm sure there is more to this story -- ...

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by jonmoulton
Thu Sep 27, 2012 4:26 pm
 
Forum: Genetics
Topic: Recombinant DNA not degraded inside transformed cells, why?
Replies: 10
Views: 4857

genetic engineering- not understanding some concepts

... use PCR? I have a quiz coming up in 5 days and I was wondering if there are any good sites for solving questions about ligation, restriction endonuclease, blunt and sticky ends and pUC8 plasmids and whatnot. If you know any sites that have answers as well as questions please let me know otherwise ...

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by radioinahole
Fri Oct 21, 2011 2:38 pm
 
Forum: Molecular Biology
Topic: genetic engineering- not understanding some concepts
Replies: 2
Views: 1825
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