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End plate

End plate

(Science: anatomy, physiology) The area of sarcolemma immediately below the synaptic region of the motor neuron in a neuromuscular junction. most often refers to the end of a nerve fibre against a skeletal muscle fibre, to which it transmits nerve signals.


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Swarming Assay

Hi, I have a question considering a swarming assay on E.coli... I did a soft agar (0.3%), in LB, and placed a drop of bacteria in the middle of a plate. But after 24h, they just grew in a small radius, like in the photo: http://i.imgur.com/gwYGLZE.png DO you think I have non-motile ...

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by SpellForce
Wed Oct 29, 2014 8:21 pm
 
Forum: Microbiology
Topic: Swarming Assay
Replies: 0
Views: 498

Re: Lonza MycoAlert test

This assay is quite robust but like all products has a few pitfalls. Questions: 1.) are you using a multifunction plate reader with a luminescence setting or a dedicated cuvette luminometer? 2.) Are you using MycoAlert or MycoAlert Plus? Some multifunction plate readers have hopelessly ...

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by buzzbomb711
Fri Jul 25, 2014 6:51 am
 
Forum: General Discussion
Topic: Lonza MycoAlert test
Replies: 1
Views: 2452

cell plate formation

During the division of plant cell, the cell plate form exactly or approximately at the center of dividing cell. What process position cell plate to the center of the cell.

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by josem
Wed Jul 23, 2014 7:14 pm
 
Forum: Cell Biology
Topic: cell plate formation
Replies: 0
Views: 1647

centrifugation of hemocytes

... a invertebrates. Many articles perform centrifugation steps after collecting the hemolymph, whether in eppenddorf, whether directly on a 96-well plate. Theses cells are circulating blood cells but display quite good adherence. Here is my problem: after centrifugation ( I tried 5 min at 300g, ...

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by Thom
Mon Jan 27, 2014 6:41 pm
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 5195

Re: blank and substrate control give OD reads

You centrifuge the reactions in the 96-well plate and then in that you measure absorbance? How can you do that if the bottom is blocked by your substrate? Can you describe your reactions and controls in more detail including some example absorbances ...

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by intandiana
Sun Jan 19, 2014 5:57 am
 
Forum: Molecular Biology
Topic: blank and substrate control give OD reads
Replies: 9
Views: 6610
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