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End-tidal

End-tidal

at the end of a normal expiration.


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Unidentified Non-Human Remain

... the posterior of the bone (for purposes of identifying, it is not to suggest an orientation), is very smooth. The sharp point that appears on one end is simply a result of post-mortem breakage and could represent a large feature that has been lost. This remain in particular was found in Colorado. ...

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by JeremiahRocker
Thu Feb 28, 2013 6:15 pm
 
Forum: Zoology Discussion
Topic: Unidentified Non-Human Remain
Replies: 0
Views: 71

TCA preipitation

... (haven't done before) but I assume it's too isolate the proteins within the cell wall?? He told me I would have three components by the end of it, the soluble, insoluble and something else!!! (the isolated proteins??) arrg I have looked at a protocol for a cell wall extraction. Here ...

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by biology_06er
Wed Feb 27, 2013 3:25 am
 
Forum: General Discussion
Topic: TCA preipitation
Replies: 2
Views: 167

How to prepare a 3.5% agarose gel ?

I cooked it on a hot plate on low temp with a stirring magnet and in the end put it in a microwave oven on low-medium for a couple of soconds a few times to get rid of the bubbles. and after getting a clear solution i added the atidium bromide. worked great.

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by itamo
Sun Feb 17, 2013 5:21 am
 
Forum: Molecular Biology
Topic: How to prepare a 3.5% agarose gel ?
Replies: 4
Views: 387

MOLECULAR CLONING

... I understand, it is not necessary to dephosphorylate my double-digested open vector as long as it was cut with two different enzymes. Thus, the 5'-end of my vector has a phosphate. But still, should I phosphorylate my insert, since its 5'-end does not have a phosphate...?

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by kk
Tue Feb 12, 2013 2:48 pm
 
Forum: Molecular Biology
Topic: MOLECULAR CLONING
Replies: 7
Views: 1513

Re: DHP, emotional cycles, food affecting emotions?

... or just get more concentrated? unlike me.. Heh, uh, anyways, That's question number one. 'Cause what if I decide to lose weight some day, and I end up affecting my future children? ..If I don't die in a freak car accident... Yeah, that's um- if you know the answer to that, I would be totally ...

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by Ahsmeah
Fri Feb 08, 2013 11:37 pm
 
Forum: General Discussion
Topic: DHP, emotional cycles, food affecting emotions?
Replies: 2
Views: 170
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