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Dictionary » E » End-diastolic volume

End-diastolic volume

End-diastolic volume

The amount of blood in the ventricle immediately before a cardiac contraction begins; a measurement of cardiac filling between beats, related to diastolic function.


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Problems making chemically competent cells

... the centrifuge and its rotor • Work in the cold room! • Pre-chill eppendorfs in the -70 freezer, and tips in the cold room, (and maybe an eppendorf ... 100ml pre-warmed SOC medium with 5ml of the overnight pre-culture. The volume of the flask here is important for aeration. I use a 2 litre baffled ...

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by lilruthc
Fri Feb 08, 2013 1:09 pm
 
Forum: Molecular Biology
Topic: Problems making chemically competent cells
Replies: 1
Views: 1843

Resuspending bacterial pellet to a certain OD600

... I plan to do some flow next week and an one of the steps is to resuspend the bacterial pellet to an OD600 0.5. So say I had a 1.5mL overnight ... you a concentration factor (cf) Divide the original culture volume (1.5mL) by the cf 1.5/0.29=5.1mL Ok..so I don't need 5.1mL of culture ...

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by biology_06er
Fri Jun 29, 2012 2:23 am
 
Forum: General Discussion
Topic: Resuspending bacterial pellet to a certain OD600
Replies: 8
Views: 2882

DNA extraction forms very a viscous substance

... mucus like substance results. If I carry on the protocol as recomended by Quiagen and add the AP2 buffer (precipitation buffer), centrifuge ... or no success. The alterations are mostly related to alterations in the volume of AP1 and/or AP2 used during the extraction. Does anyone have similar ...

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by calobo
Tue Jan 10, 2012 5:21 pm
 
Forum: Molecular Biology
Topic: DNA extraction forms very a viscous substance
Replies: 1
Views: 1219

Hard time with homework question

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by daniel.kurz
Mon Nov 21, 2011 9:48 pm
 
Forum: General Discussion
Topic: Hard time with homework question
Replies: 6
Views: 3983

Re: drink water, orange juice or Cola before eating?

... all proteins are enzymes) such as trypsin start off with -ogen at the end of their name. Pepsinogen and trypsinogen. They are cleaved by the hydrochloric ... be a major problem for those that suffer hypertension and other blood volume conditions. The stomach cannot mix properly if it does not have ...

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by daniel.kurz
Mon Oct 24, 2011 12:26 am
 
Forum: Physiology
Topic: drink water, orange juice or Cola before eating?
Replies: 15
Views: 32103
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