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Embryos

Embryo sac

(Science: botany) see embryonic.


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Two-color in situ help

Thanks for the reply. I looked at whole embryos and the auto-fluorescence was so weak that it wouldn't even be picked up by our most sensitive cameras. I also suspected the endogenous peroxidase activity but that did not seem to factor in either ...

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by vastgenome
Thu Feb 14, 2013 11:59 pm
 
Forum: Molecular Biology
Topic: Two-color in situ help
Replies: 3
Views: 259

Re: aneuploidy and polyploidy

... in sex chromosomes or autosomes...AND there are different causes (non-disjunction, translocation errors, mitosis separation failures in early embryos). Plus there are a whole range of syndromes associated with each of these types (i.e. Jacob Syndrome is an aneuploid condition in the sex chromosomes ...

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by carmodyf
Thu Oct 11, 2012 4:36 am
 
Forum: Cell Biology
Topic: aneuploidy and polyploidy
Replies: 2
Views: 1395

PCR from cDNA question

... been experiencing many weird problems so I hope the brilliant minds here can shed some light on it. The total RNA was extracted from ~100 whole embryos (~1000 cells each) of an invertebrate marine species using QIAGEN RNeasy Plus mini kit eluted with 40ul RNase free water. Nanodrop gives a low ...

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by vastgenome
Fri Jun 22, 2012 3:55 pm
 
Forum: Molecular Biology
Topic: PCR from cDNA question
Replies: 1
Views: 1647

microRNA in situ hybridization protocol: zebrafish

... fine work on this one. Lagendijk AK, Moulton JD, Bakkers J. Revealing details: whole mount microRNA in situ hybridization protocol for zebrafish embryos and adult tissues. Biology Open. 2012;[Advance Online Publication] doi:10.1242/bio.2012810 http://bio.biologists.org/content/early/2012/04/11/bio.2012810.full

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by jonmoulton
Fri Apr 27, 2012 4:32 pm
 
Forum: Molecular Biology
Topic: microRNA in situ hybridization protocol: zebrafish
Replies: 0
Views: 526

Re: The Most Primitive Respiratory System

chikis "The embryos (the earliest stage of and development of both plants and animals) of fish, reptile, birds and mammals are very similar and this are evidence that they evolved from a distant common ancestor. Embroyology shows ...

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by JorgeLobo
Fri Apr 27, 2012 1:39 am
 
Forum: Evolution
Topic: The Most Primitive Respiratory System
Replies: 11
Views: 2537
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