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Emb agar

Emb agar --> eosin-methylene blue agar

agar composed of peptone, lactose, and sucrose and containing eosin and methylene blue, used to distinguish between lactose-fermenting and non-lactose-fermenting gram-negative bacteria.

Synonym: emb agar.


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How would I do this experiment? [Advanced]

... you cannot perform the test at all), it would be better to perform dilution of your samples and spread aliquots of such on plates with growth agar (for E.coli simple LB, for Kefir, no idea). Let them growth (E.coli for 1 day at 37°C) and count number of colonies (pick plates with 10-300 colonies, ...

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by JackBean
Fri Dec 19, 2014 2:46 pm
 
Forum: Cell Biology
Topic: How would I do this experiment? [Advanced]
Replies: 2
Views: 1024

Beginner Microbiologist, looking for a place to start.

... recently purchased a lab microscope, and a ton of lab supplies. I have done all the basics- looked at pond water, plants, I have grown bacteria in Agar plates. I am at a point where I want to start doing experiments or research that contribute to science in some way. Does anyone have any ideas? ...

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by fstevens
Sat Dec 13, 2014 3:30 pm
 
Forum: General Discussion
Topic: Beginner Microbiologist, looking for a place to start.
Replies: 1
Views: 1070

Starch Hydrolysis

Normal RECIPE for STRACH Hydrolysis: Starch agar composition (g/liter) Beef extract 3 g Soluble starch 10 g Agar 12 g Distilled water 1 liter But should we can use Peptone instead of Beef extract ?

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by Helics
Sun Nov 30, 2014 10:00 am
 
Forum: Microbiology
Topic: Starch Hydrolysis
Replies: 0
Views: 538

Swarming Assay

Hi, I have a question considering a swarming assay on E.coli... I did a soft agar (0.3%), in LB, and placed a drop of bacteria in the middle of a plate. But after 24h, they just grew in a small radius, like in the photo: http://i.imgur.com/gwYGLZE.png ...

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by SpellForce
Wed Oct 29, 2014 8:21 pm
 
Forum: Microbiology
Topic: Swarming Assay
Replies: 0
Views: 452

Re:

... is not very specific, and telling me to speak with someone else isn't very helpful either... I think you can use a test, where semi-solid agar taken in test tube, which will a gradation of oxygen concentration. Surface have normal oxygen concentration and when we go downwards its concentration ...

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by sosippus
Wed Sep 24, 2014 7:44 pm
 
Forum: Microbiology
Topic: Oxygen requirements of eukaryotes
Replies: 7
Views: 4451
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