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Dictionary » E » Elution volume

Elution volume

Definition

noun

(1) The volume of the eluent or buffering agent that elutes from the column of a chromatograph before a particular peak in the elution profile appears (meaning before a particular substance of interest comes out with the eluent or buffer).

(2) The volume of solvent eluting from the column over the period, from the time of the sample injection to the time when the maximum concentration per unit time is eluting from the column.


Supplement

See also: elution profile


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Re:

if you're using just water for elution, you should check pH of the water. Also, I think, that let it stand for at ... The other thing, you can do two elutions (even with the same water). BTW what volume did you use? Do you mean the system with the filters, right? So, it should be ...

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by vastgenome
Mon Apr 12, 2010 5:40 am
 
Forum: Molecular Biology
Topic: RNA prep help
Replies: 4
Views: 2556

RNA prep help

if you're using just water for elution, you should check pH of the water. Also, I think, that let it stand for at ... The other thing, you can do two elutions (even with the same water). BTW what volume did you use? Do you mean the system with the filters, right? So, it should be ...

See entire post
by JackBean
Mon Apr 05, 2010 6:04 pm
 
Forum: Molecular Biology
Topic: RNA prep help
Replies: 4
Views: 2556

Lost Protein?

... sepharose (Q). Now the problem is I'm not seeing my protein after elution, roughly about 20 times the volume of the column has been passed through (again, 0.1M to 1.0M). The protein was supposed ...

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by BrianScott
Tue Jun 30, 2009 4:05 pm
 
Forum: Molecular Biology
Topic: Lost Protein?
Replies: 0
Views: 1878

Double restriction digest, ligation, transformation

... is also suggested in the kit. I had also very little amunt of DNA afer elution because my plasmid was bigger than 10.0000 KD ( the kit is fdesign ... However, you can cut the band as small as possible, and use a little volume for elution. I always digest my plasmid in parallel tubes and I pooled ...

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by nena77
Thu Feb 26, 2009 10:10 am
 
Forum: Molecular Biology
Topic: Double restriction digest, ligation, transformation
Replies: 1
Views: 3927


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