Dictionary » E » Elution

Elution

Definition

noun

(1) The removal or separation of one material from another, especially with a solvent.

(2) The process of extracting a substance adsorbed to another by means of a suitable solvent or buffering agent as in column chromatography.

(3) The removal or separation of antibody from the antigen to which it is attached.


Supplement

Word origin: elute » from Latin ēluere, ēlūt-, to wash out : ē-, ex-, ex- + -luere, to wash.
Related forms: elute (verb).

Synonym:

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Results from our forum


Cloning/Transformation question

The proper way to make negative control is to set up a ligation of your digested vector with water (or TE buffer/elution buffer you are using) in place of insert DNA and use that ligation as negative control in transformation. That gives you an idea of the background (self-ligated ...

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by Cat
Sat Dec 01, 2012 12:50 am
 
Forum: Molecular Biology
Topic: Cloning/Transformation question
Replies: 4
Views: 1921

Re: dna purity is 17? what does it mean?

... first time ı see a value like this. But ı make dna cleaning and concentrating prosedure and forget my dna sample 5 or 7 minutes in the final step(elution step). Is this the matter I really don't undestand??

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by genetherapy
Fri Jan 27, 2012 6:48 am
 
Forum: Molecular Biology
Topic: dna purity is 17? what does it mean?
Replies: 6
Views: 2184

Cobalt turning orange ??

... vanishes : it's there in the soluble extract that i load on the column, but not anymore after the column, neither in the flow through or the elution... I've been then trying the old batch method... In parallel, with Talon resin (cobalt) and Ni-NTA resin (nickel). What was weird, was a change ...

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by mgn67
Sun Jan 22, 2012 11:32 am
 
Forum: Molecular Biology
Topic: Cobalt turning orange ??
Replies: 0
Views: 887

Re: T7 Expression System

... an in-vivo method to try. I've noticed here: http://www.sciencedirect.com/science/article/pii/0014579383806243 The use of blue-sephadex, Ap5A elution and a second G-100 sephadex separation yield homogenous adenylate kinase in E.Coli anyway (the putative gene i'm dealing with show's a high ...

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by TjCooper
Tue Jan 03, 2012 1:22 pm
 
Forum: Molecular Biology
Topic: T7 Expression System
Replies: 5
Views: 2979

Re:

Well from what I understand, 66mM of imidazole was enough to elute your protein so you do not need to add more, and you can use your elution to do all your experiments further. I would agree, but he wrote, that the first time, he had the protein also in franctions 80 and 100 mM

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by JackBean
Mon Oct 31, 2011 11:11 am
 
Forum: Molecular Biology
Topic: Wrong imidazole conc
Replies: 3
Views: 1365
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