Dictionary » E » Elution




(1) The removal or separation of one material from another, especially with a solvent.

(2) The process of extracting a substance adsorbed to another by means of a suitable solvent or buffering agent as in column chromatography.

(3) The removal or separation of antibody from the antigen to which it is attached.


Word origin: elute » from Latin ēluere, ēlūt-, to wash out : ē-, ex-, ex- + -luere, to wash.
Related forms: elute (verb).


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Results from our forum

Plasmid yield/concentration

... usually results in high plasmid concentration in the final DNA preparation. Alternatively, larger volume of stating material to smaller volume elution can be used to concentrate low yield plasmid prep...

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by Cat
Sat Sep 20, 2014 4:18 pm
Forum: Molecular Biology
Topic: Plasmid yield/concentration
Replies: 3
Views: 2555

Cloning/Transformation question

The proper way to make negative control is to set up a ligation of your digested vector with water (or TE buffer/elution buffer you are using) in place of insert DNA and use that ligation as negative control in transformation. That gives you an idea of the background (self-ligated ...

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by Cat
Sat Dec 01, 2012 12:50 am
Forum: Molecular Biology
Topic: Cloning/Transformation question
Replies: 4
Views: 2868

Re: dna purity is 17? what does it mean?

... first time ı see a value like this. But ı make dna cleaning and concentrating prosedure and forget my dna sample 5 or 7 minutes in the final step(elution step). Is this the matter I really don't undestand??

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by genetherapy
Fri Jan 27, 2012 6:48 am
Forum: Molecular Biology
Topic: dna purity is 17? what does it mean?
Replies: 6
Views: 3280

Cobalt turning orange ??

... vanishes : it's there in the soluble extract that i load on the column, but not anymore after the column, neither in the flow through or the elution... I've been then trying the old batch method... In parallel, with Talon resin (cobalt) and Ni-NTA resin (nickel). What was weird, was a change ...

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by mgn67
Sun Jan 22, 2012 11:32 am
Forum: Molecular Biology
Topic: Cobalt turning orange ??
Replies: 0
Views: 1273

Re: T7 Expression System

... an in-vivo method to try. I've noticed here: http://www.sciencedirect.com/science/article/pii/0014579383806243 The use of blue-sephadex, Ap5A elution and a second G-100 sephadex separation yield homogenous adenylate kinase in E.Coli anyway (the putative gene i'm dealing with show's a high ...

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by TjCooper
Tue Jan 03, 2012 1:22 pm
Forum: Molecular Biology
Topic: T7 Expression System
Replies: 5
Views: 4195
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