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Dictionary » E » Elute

Elute

Definition

verb

To cause elution; to separate, remove, or extract a particular substance from another, usually by means of a solvent.


Supplement

Word origin: from Latin ēluere, ēlūt-, to wash out : ē-, ex-, ex- + -luere, to wash.
Related forms: elution (noun), eluent (noun)

Synonym:


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Re: HCL instead of imidazole!

Hi, I'm using a nickel column, when i added my reducing buffer to a sample of beads and my eluted protein it turned yellow..so i washed it with my buffer and then tested for colour change...it didn't turn yellow...so i eluted with 100mM of imadazole and ran a gel and ...

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by biology_06er
Fri Dec 16, 2011 9:23 am
 
Forum: General Discussion
Topic: HCL instead of imidazole!
Replies: 6
Views: 1120

HCL instead of imidazole!

1M HCl has pH ~1, that denatured probably your protein and I'm not sure, whether it doesn't elute also the ion from column. Which ion are you using? Did you see decoloration of your column? Do you have protein of which you can measure activity? If so, do it and you will ...

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by JackBean
Fri Dec 16, 2011 7:18 am
 
Forum: General Discussion
Topic: HCL instead of imidazole!
Replies: 6
Views: 1120

Re: thin layer chromatography

If you've easy access to a UV-Vis spec, you could scrape off the bands, elute them and take some spectra -- then assign the kids into the literature to find likely candidates. It could get complicated if some pigments are co-migrating, but it would also be fun ...

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by jonmoulton
Mon Nov 28, 2011 10:45 pm
 
Forum: Molecular Biology
Topic: thin layer chromatography
Replies: 4
Views: 1296

Re:

Well from what I understand, 66mM of imidazole was enough to elute your protein so you do not need to add more, and you can use your elution to do all your experiments further. I would agree, but he wrote, that the first time, he had the protein also in ...

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by JackBean
Mon Oct 31, 2011 11:11 am
 
Forum: Molecular Biology
Topic: Wrong imidazole conc
Replies: 3
Views: 968

Wrong imidazole conc

Well from what I understand, 66mM of imidazole was enough to elute your protein so you do not need to add more, and you can use your elution to do all your experiments further. NAd next time you can use any concentration >66mM to elute your protein. ...

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by canalon
Mon Oct 31, 2011 10:04 am
 
Forum: Molecular Biology
Topic: Wrong imidazole conc
Replies: 3
Views: 968
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