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Dictionary » E » Eluent

Eluent

Definition

noun, plural: eluents

A substance that separates and moves constituents of a mixture through the column of a chromatograph.


Supplement

The eluent in liquid chromatography is a liquid solvent whereas in gas chromatography is a carrier gas.


Word origin: Latin ēluēns, ēluent-, present participle of ēluere, to wash out
Related forms: elution (noun), eluate (verb)

Variant: eluant

Synonym: elutant

Compare: eluate
See also: elution.


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Re: doubt about Detoxi-Gel™ Endotoxin Removing Gel

... in the column, that is, it is the volume, when comes out the non-released fraction. So, you do not have to care about the first 0.3-0.5 ml of eluent, because there is only the buffer. The bed volume is the total volume of the column, including the matrice, so you can actually calculate it ...

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by JackBean
Fri Jan 08, 2010 8:33 am
 
Forum: Cell Biology
Topic: doubt about Detoxi-Gel™ Endotoxin Removing Gel
Replies: 7
Views: 2699

gel extraction problem

Maybe the time is not long enough when you incubate the washed DNA with eluent buffer. In my lab, most staffs here use QiaGen QiaQuick Column and mostly they succeed in DNA extraction from gel.

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by victor
Wed Jul 16, 2008 1:53 am
 
Forum: Molecular Biology
Topic: gel extraction problem
Replies: 4
Views: 2496


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