Dictionary » E » Eluate

Eluate

Definition

noun, plural: eluates

(1) The liquid or solution of solvent and dissolved material produced during elution or chromatography.

(2) The substance separated out or emerging from the column of a chromatograph.


Supplement

Word origin: Latin ─ôlu(ere) to wash out.

See also: chromatography, [[elution ]]


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Removing MBP tag

... if your protein is smaller than the MBP it or use a selection of Amicon filters (or something similar) in cascade to 1st get your protein in the eluate, and then wash it up in a filter that would keep the protein the saved fraction.

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by canalon
Tue Aug 16, 2011 5:52 pm
 
Forum: Molecular Biology
Topic: Removing MBP tag
Replies: 12
Views: 8700

Re: inactive enzyme

The easiest way to get rid of imidazole is either to dialyze it away, or dilute the Ni column eluate with buffer and do an ion-exchange run as a next step in the purification. It may also be that your protein doesn't like phosphate buffer, or that metal ions are leaching ...

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by blcr11
Thu Feb 14, 2008 11:38 am
 
Forum: Molecular Biology
Topic: inactive enzyme
Replies: 3
Views: 2267


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