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Elisa

Elisa --> enzyme-linked immunoabsorbent assay

(Science: investigation) The enzyme-linked immunoabsorbent assay is serologic test used as a general screening tool for the detection of antibodies to the hiv virus. Reported as positive or negative. Since false positive tests due occur (for example recent flu shot), positives will require further evaluation using the western blot. Elisa technology links an a measurable enzyme to either an antigen or antibody. In this way, it can then measure the presence of an antibody or an antigen in the bloodstream.

Acronym: elisa


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Antibody Coating

Try this one: http://www.piercenet.com/files/TR0065-ELISA-guide.pdf

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by Cat
Sun Aug 18, 2013 4:51 pm
 
Forum: Microbiology
Topic: Antibody Coating
Replies: 1
Views: 978

ELISA

ELISA protocols changes according to the spesific antigen or the antibody investigated. It also changes according the antigen or antibody whether you use monoclonal or policlonal. What are you investigating specifically? And ...

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by gislab03
Sat May 04, 2013 2:11 pm
 
Forum: Molecular Biology
Topic: ELISA
Replies: 1
Views: 1244

Re: Protein isolation

Okay, after doing some reading, I have come up with a different plan for quantifying HIF-1 alpha, which involves running an ELISA after performing a nuclear extraction. However, all the protocols and kits I have seen recommend 1-10 million cells for a good extraction. I am likely going ...

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by roth317
Tue Feb 19, 2013 7:28 pm
 
Forum: Molecular Biology
Topic: Protein isolation
Replies: 2
Views: 1946

ELISA

Does anyone have a protocol for ELISA? Thanks

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by Bio2013
Sat Feb 16, 2013 8:50 pm
 
Forum: Molecular Biology
Topic: ELISA
Replies: 1
Views: 1244

Protein isolation

... (I am looking at cell aggregates to be specific). Our original plan is to isolate the protein using trizol extraction reagent, then run an ELISA on HIF 1 alpha. However, I am worried that the protocol may not work. I haven't heard of trizol being used for a specific protein extraction, ...

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by roth317
Tue Feb 12, 2013 9:25 pm
 
Forum: Molecular Biology
Topic: Protein isolation
Replies: 2
Views: 1946
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