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Electrophoretic

Electrophoretic

Relating to electrophoresis, as an electrophoretic separation.

Synonym: ionophoretic.


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electrophoretic mobility shift assay (EMSA)

I am doing EMSA and after the first try having success, I could never reproduce my results: following the streptavidin-biotin-HRP detection, there are no bands on the blot paper. Compared to the first succesful try, the most striking difference I experience is that while at first, there was a bromop...

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by kk
Fri Nov 08, 2013 12:21 pm
 
Forum: Molecular Biology
Topic: electrophoretic mobility shift assay (EMSA)
Replies: 1
Views: 1165

Re: Gene Technology

... the blue pigment because its substrate (lilac pigment) is not present. In the second row, both enzymes are present (both bands appeared in the electrophoretic lane). This means enzyme 1 is present and can catalyze the formation of the lilac pigment from its uncolored precursor. Enzyme 2 is ...

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by jonmoulton
Tue Apr 10, 2012 3:57 pm
 
Forum: Genetics
Topic: Gene Technology
Replies: 1
Views: 1759

Help Needed - Ligation Pairing (Vector and DNA)

... DNA? If you had, is there a step in this series of experiments that would have prevented the propagation of the incorrect plasmid? 3. Did the electrophoretic pattern of your uncut recombinant plasmid contain many forms of DNA like your ligation reaction? Explain. 4. Can the size of a supercoiled ...

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by techbrit
Sun Mar 13, 2011 7:34 pm
 
Forum: Molecular Biology
Topic: Help Needed - Ligation Pairing (Vector and DNA)
Replies: 0
Views: 1799

SDS-PAGE

... relative mobility is calculated by the distance moved by a protein standard divided by the distance moved by bromophenol blue (a marker of the electrophoretic front). how do i graph this: on the y-axis do i just log the molecular weights (from 10kd to 250kd) and then plot it against what??...im ...

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by biology_06er
Mon Mar 23, 2009 3:58 am
 
Forum: Molecular Biology
Topic: SDS-PAGE
Replies: 2
Views: 7096

Re:

... 6. Elute protein/DNA mixture - research conditions for binding and washing to make sure not to affect protein-DNA interactions. 7. Proceed with electrophoretic mobility shift assay AND/OR isolate DNA, clone, and sequence. 8. Let us know the outcome. Good luck! Thank you for the suggestion. I ...

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by evilid
Mon Dec 01, 2008 10:19 pm
 
Forum: Molecular Biology
Topic: Does my protein bind DNA?
Replies: 12
Views: 9312
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