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Electrophoresis

Electrophoresis

(Science: technique) separation of ionic molecules, (principally proteins) by the differential migration through a gel according to the size and ionic charge of the molecules in an electrical field. High resolution techniques normally use a gel support for the fluid phase.

Examples of gels used are starch, acrylamide, agarose or mixtures of acrylamide and agarose. Frictional resistance produced by the support causes size, rather than charge alone, to become the major determinant of separation.

Smaller molecules with a more negative charge will travel faster and further through the gel toward the anode of an electrophoretic cell when high voltage is applied. Similar molecules will group on the gel. They may be visualised by staining and quantitated, in relative terms, using densitometers which continuously monitor the photometric density of the resulting stain.

The electrolyte may be continuous (a single buffer) or discontinuous, where a sample is stacked by means of a buffer discontinuity, before it enters the running gel/ running buffer. The gel may be a single concentration or gradient in which pore size decreases with migration distance.

in sds gel electrophoresis of proteins or electrophoresis of polynucleotides, mobility depends primarily on size and is used to determined molecular weight. In pulse field electrophoresis, two fields are applied alternately at right angles to each other to minimise diffusion mediated spread of large linear polymers.

See: electrofocussing, pulse field electrophoresis


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Re: Gel electrophoresis

haha, so there is a high concentration of the small fragments?

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by downingrock
Thu Mar 13, 2014 1:08 am
 
Forum: Genetics
Topic: Gel electrophoresis
Replies: 3
Views: 1591

Gel electrophoresis

Hey guys! Would anyone know, in each of the Lane 2's, why there is a very large bright fragment that doesn't really confine to the shape of a rectangle? Just a general description would be helpful. Thank you!

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by downingrock
Wed Mar 12, 2014 12:44 pm
 
Forum: Genetics
Topic: Gel electrophoresis
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plasmid extraction on 1.5% agarose gel

http://www.bioinformatics.nl/molbi/SimpleCloningLab/electrophoresis.htm check the part about plasmid DNA

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by JackBean
Thu Dec 26, 2013 5:56 pm
 
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by Nevthonien
Tue Nov 12, 2013 2:47 pm
 
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by namakulaila
Thu Oct 17, 2013 6:17 am
 
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