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Efficiency

Efficiency

1. The quality of being efficient or producing an effect or effects; efficient power; effectual agency. The manner of this divine efficiency being far above us. (Hooker)

2. (Science: mechanics) The ratio of useful work to energy expended. Efficiency of a heat engine, the ratio of the work done an engine, to the work due to the heat supplied to it.

Origin: L. Efficientia.


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MolBio calculations

... to fin. volume 5 ml (i.e. 10×). From that you take 50 ul, which is 1/100th of 5 ml, i.e. 2.5 ng of DNA. and this gave 256 colonies. thus you have efficiency about 100 colonies per 1 ng of DNA if I calculate correctly.

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by JackBean
Wed Dec 04, 2013 8:12 pm
 
Forum: Molecular Biology
Topic: MolBio calculations
Replies: 1
Views: 870

qPCR vs Northern Blot

... stable cell line, silencing by shRNA). Firstly I tried to check if silencing works by northern blot. It looked that my silencing has around 80% efficiency. Later I wanted to confirm this result by qPCR - and then surprise - no silencing (or max around 20% efficiency). Primers had efficiency ...

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by Nevthonien
Thu Nov 07, 2013 8:35 pm
 
Forum: Molecular Biology
Topic: qPCR vs Northern Blot
Replies: 4
Views: 1285

How do I Calculate the productivity P in KJ?

... Respiration P: Production (Production=Assimmilation-Respiration and Production = Intake - Feces - Respiration) A certain animal has a production efficiency (P/A) of 2%, while his assimilation efficiency is 80%. 1. How do I Calculate the productivity P in KJ if this animal has an energy content ...

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by DarDerk
Tue Nov 05, 2013 8:43 pm
 
Forum: Ecology
Topic: How do I Calculate the productivity P in KJ?
Replies: 0
Views: 458

Gluconeogenesis

... enzymes or lesser efficient isoforms, subcellular oragnaells than liver. The extra energy spent for nitrogen removal is the "gap" in efficiency. The nitrogen build up is more toxic to cells, so the cycle of alanine to glucose is very difficult. kidney < liver

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by balamurugan3
Wed Aug 07, 2013 9:57 pm
 
Forum: Cell Biology
Topic: Gluconeogenesis
Replies: 8
Views: 5211

cDNA cloning and Lentiviral transfection

... this vector for cloning in bacteria? Else, where do I start? Also, I am considering lentiviral transduction of my mammalian cells as it has better efficiency. Is there a different method/ plasmids I need to use during cloning to be able to perform lentiviral transduction in later stages? Any advice ...

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by rkrishna
Thu Jun 27, 2013 11:22 pm
 
Forum: Molecular Biology
Topic: cDNA cloning and Lentiviral transfection
Replies: 1
Views: 556
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