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Edta-atpase

Edta-atpase

(Science: enzyme) aspect of EC 3.6.1.3

registry number: EC 3.6.1.-

Synonym: atpase, edta, k_(edta) atpase, edta-k_-atpase


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centrifugation of hemocytes

I'm not expert in animal biology, but I'd say you do not need EDTA or heparin for resuspension, since the cells are pelleted because of centrifugation, not because they grew there. What about trying some Western or other protein detection after the resuspension ...

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by JackBean
Thu Jan 30, 2014 9:34 am
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 2415

centrifugation of hemocytes

... to be "pelleted" to the bottom of the wells and so when i wash, the cells should stay sticked, unless i resuspend with PBS containing EDTA or heparin... Is it correct? When i take off the medium after centrifugation, and then add PBS are the cell straightforward resuspended or can ...

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by Thom
Tue Jan 28, 2014 10:50 am
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 2415

Culturing or viewing cells

Insuline is better than EDTA. You much quickly spin down the blood cell, otherwise it will have hemolysis. ___________________________________________________________________________________________ http://www.agenombio.com

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by agb06
Fri Sep 13, 2013 2:21 am
 
Forum: Cell Biology
Topic: Culturing or viewing cells
Replies: 3
Views: 3183

Culturing or viewing cells

I wish I could answer every questions but not... 1. collect blood always with EDTA, which chelates Calcium and prevents cloting. other answers need some experts...

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by balamurugan3
Sat Aug 10, 2013 3:17 am
 
Forum: Cell Biology
Topic: Culturing or viewing cells
Replies: 3
Views: 3183

Chromatin experiment

... 6 6= wrong interpretation 7= lane 3 Which of the following protocols modifications could remove the high molecular weight bands? 1. Increase the EDTA concentration 2. Expose the cells to the detergent for longer time 3. Increase the concentration of the nuclease 4. Decrease the concentration ...

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by 2468
Thu Nov 08, 2012 7:48 pm
 
Forum: Molecular Biology
Topic: Chromatin experiment
Replies: 10
Views: 3102
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