Edta

... 6 6= wrong interpretation 7= lane 3 Which of the following protocols modifications could remove the high molecular weight bands? 1. Increase the EDTA concentration 2. Expose the cells to the detergent for longer time 3. Increase the concentration of the nuclease 4. Decrease the concentration ...

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... Or do they have mini-scrapers for so small wells? You could try seeding the cells on a bigger plate and using plastic cell scrapers after the EDTA-treatment. I have used 5 mM EDTA in PBS (pH 7.2) and incubated my cells for some 5 to 15 mins in +4C with moderate success (some of the cells tend ...

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... represent the molecular weight standards Lane 3 = 7. The lack of bands in this lane (except for a very faint band at at 200bp) demonstrates that EDTA has effectively inhibited the nuclease enzyme Thank you! :)

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... represent the molecular weight standards Lane 3 = 7. The lack of bands in this lane (except for a very faint band at at 200bp) demonstrates that EDTA has effectively inhibited the nuclease enzyme

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Indeed want to study monocytes. I think I'm going to use PBS/edta and to scrap them with the tips of the pipette. Do you think it's a good idea?

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