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Edta

Edta --> ethylene diamine tetra acetate

(Science: chemical) a chemical that is used to remove all traces of magnesium and calcium ions from a solution because it binds tightly to them, in order to control unwanted side reactions with these metals during a laboratory process.

Acronym: edta


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Chromatin experiment

... 6 6= wrong interpretation 7= lane 3 Which of the following protocols modifications could remove the high molecular weight bands? 1. Increase the EDTA concentration 2. Expose the cells to the detergent for longer time 3. Increase the concentration of the nuclease 4. Decrease the concentration ...

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by 2468
Thu Nov 08, 2012 7:48 pm
 
Forum: Molecular Biology
Topic: Chromatin experiment
Replies: 10
Views: 998

peripheral mononuclear cells handling

... Or do they have mini-scrapers for so small wells? You could try seeding the cells on a bigger plate and using plastic cell scrapers after the EDTA-treatment. I have used 5 mM EDTA in PBS (pH 7.2) and incubated my cells for some 5 to 15 mins in +4C with moderate success (some of the cells tend ...

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by biohazard
Mon Nov 05, 2012 1:11 pm
 
Forum: Cell Biology
Topic: peripheral mononuclear cells handling
Replies: 3
Views: 688

Re:

... represent the molecular weight standards Lane 3 = 7. The lack of bands in this lane (except for a very faint band at at 200bp) demonstrates that EDTA has effectively inhibited the nuclease enzyme Thank you! :)

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by 2468
Sat Nov 03, 2012 4:32 pm
 
Forum: Molecular Biology
Topic: Chromatin experiment
Replies: 10
Views: 998

Chromatin experiment

... represent the molecular weight standards Lane 3 = 7. The lack of bands in this lane (except for a very faint band at at 200bp) demonstrates that EDTA has effectively inhibited the nuclease enzyme

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by Cat
Sat Nov 03, 2012 3:46 pm
 
Forum: Molecular Biology
Topic: Chromatin experiment
Replies: 10
Views: 998

Re: peripheral mononuclear cells handling

Indeed want to study monocytes. I think I'm going to use PBS/edta and to scrap them with the tips of the pipette. Do you think it's a good idea?

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by claire84
Fri Nov 02, 2012 2:56 pm
 
Forum: Cell Biology
Topic: peripheral mononuclear cells handling
Replies: 3
Views: 688
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