Dictionary » E » Edta

Edta

Edta --> ethylene diamine tetra acetate

(Science: chemical) a chemical that is used to remove all traces of magnesium and calcium ions from a solution because it binds tightly to them, in order to control unwanted side reactions with these metals during a laboratory process.

Acronym: edta


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


use of a bacterial protease inhibitor cocktail with mammalia

... and protease inhibitor cocktail. A Millipore technical representative recommended my using "539134 | Protease Inhibitor Cocktail Set III, EDTA-Free - Calbiochem" as a good general protease inhibitor cocktail. I have another protease inhibitor cocktail which includes: AEBSF, bestatin, ...

See entire post
by rpwdoc
Tue Aug 26, 2014 10:08 pm
 
Forum: Cell Biology
Topic: use of a bacterial protease inhibitor cocktail with mammalia
Replies: 0
Views: 99

centrifugation of hemocytes

I'm not expert in animal biology, but I'd say you do not need EDTA or heparin for resuspension, since the cells are pelleted because of centrifugation, not because they grew there. What about trying some Western or other protein detection after the resuspension ...

See entire post
by JackBean
Thu Jan 30, 2014 9:34 am
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 2602

centrifugation of hemocytes

... to be "pelleted" to the bottom of the wells and so when i wash, the cells should stay sticked, unless i resuspend with PBS containing EDTA or heparin... Is it correct? When i take off the medium after centrifugation, and then add PBS are the cell straightforward resuspended or can ...

See entire post
by Thom
Tue Jan 28, 2014 10:50 am
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 2602

Culturing or viewing cells

Insuline is better than EDTA. You much quickly spin down the blood cell, otherwise it will have hemolysis. ___________________________________________________________________________________________ http://www.agenombio.com

See entire post
by agb06
Fri Sep 13, 2013 2:21 am
 
Forum: Cell Biology
Topic: Culturing or viewing cells
Replies: 3
Views: 3305

Culturing or viewing cells

I wish I could answer every questions but not... 1. collect blood always with EDTA, which chelates Calcium and prevents cloting. other answers need some experts...

See entire post
by balamurugan3
Sat Aug 10, 2013 3:17 am
 
Forum: Cell Biology
Topic: Culturing or viewing cells
Replies: 3
Views: 3305
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 5,189 times. 
What links here | Related changes | Permanent link