Edta

EDTA is a chemical. I guess it depends if you are discussing the protonated forms or the unprotonated forms. but for some reason, people don't seem to distinguish. i can't say really, i only use EDTA for DNA gel buffer, and ...

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My impression is that there are two kinds of EDTA, one is very acid, another is close to 7. Correct if I am wrong. You need to use the right one for your buffer.

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No when you are preparing the 0,5M EDTA you first add 80ml of MQwater to the powder, and then try to dissolve the EDTA (it is a long and painful process, but it will eventually happen) by adding NaOH to the solution. And when all the powder ...

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Thanks claudiupopa and Canalon I am wondering why to add EDTA to 80 ml of MQwater rather than 100 ml? according to the protocol, I should add 100 ml of 0.5M EDTA. So how can I put it in 80 ml?

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EDTA is annoying to dissolve to say the least. It is slightly acidic, but disssolves only at pH 8. Also I usually do not prepare my solution in PBS but in water. So take your PH meter, weigh your EDTA and add it to 80ml MQwater, ...

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