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Ecori restriction enzyme

Ecori restriction enzyme

(Science: enzyme molecular biology) a commonly-used restriction enzyme (enzyme which will cleave the phosphodiester bonds of dna at specific nucleotide sequences) that came from the bacteria escherichia coli and recognises the sequence GAATTC.

The enzyme will make a staggered cut of the double-stranded dna molecule by cutting between the g and a on both strands.


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Restriction enzyme - I think my teacher made a mistake.

... a total of 5730 bp. It has sites for the restriction enzymes SstI, EcoRI and HindIII located at the following positions: EcoRI position ...

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by TToe
Sat Nov 24, 2012 12:36 am
 
Forum: Genetics
Topic: Restriction enzyme - I think my teacher made a mistake.
Replies: 1
Views: 1505

How to draw a blot from a RFLP physical map?

... need to understant is, whether there is the restriction site or is not. That tells you the table of haplotypes.Thus haplotype A has both EcoRI sites 3 and 4, while in haplotype the site 4 was mutated and thus the enzyme is not able to cut the DNA there, thus the DNA ...

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by JackBean
Thu Nov 24, 2011 1:07 pm
 
Forum: Genetics
Topic: How to draw a blot from a RFLP physical map?
Replies: 7
Views: 3005

How to draw a blot from a RFLP physical map?

... are beneath the vertical lines ) represent restriction sites for the enzyme EcoRI.; Sites 3 and 4 are polymorphic; the others are not. The distances ...

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by ssimon10
Wed Nov 23, 2011 2:43 pm
 
Forum: Genetics
Topic: How to draw a blot from a RFLP physical map?
Replies: 7
Views: 3005

genomic rescue construct from BAC DNA

... - I cut 1.25 ug of BAC overnight with EcoRI and BamHI (the enzymes i need to generate the desired insert). The resulting ... 120 units enzyme each.> - I cut pCasper4 with the restriction enzymes for 2 hours. I gel purified the vector ...

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by GAV65
Wed Aug 24, 2011 5:08 am
 
Forum: Molecular Biology
Topic: genomic rescue construct from BAC DNA
Replies: 2
Views: 3866

Cloning with pBR322 and two restriction enzymes

... when you cut a plasmid (pBR322) with two restriction enzymes and then insert a piece of DNA to be cloned. Is ... piece from the plasmid lost? e.g. cut a plasmid with EcoRI and BamHI which are 0.4kb's apart and then insert ...

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by SamVG
Wed Apr 13, 2011 4:34 am
 
Forum: Molecular Biology
Topic: Cloning with pBR322 and two restriction enzymes
Replies: 4
Views: 4352
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