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Ecori methylase

Ecori methylase

(Science: enzyme molecular biology) An enzyme that will methylate (attach a methyl group (-CH3 group) to the nitrogenous base adenine on dna molecules) the cutting site (the sequence GAATTC) of the ecori restriction enzyme (an enzyme which will cleave dna at specific nucleotide sequences).


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EcoRI

... why E. coli's own DNA is not cut by the restriction endonuclease EcoRI? A bacterium protects its own DNA from digestion using other enzymes ... makes the restriction enzyme EcoRI and the methylating enzyme EcoRI methylase. The methylase modifies EcoRI sites in the bacteria’s own genome ...

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by kikkid
Fri Oct 05, 2007 7:30 pm
 
Forum: Genetics
Topic: EcoRI
Replies: 2
Views: 1130


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