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Eco ri

Eco ri

(Science: enzyme) probably the most commonly used type II restriction endonuclease isolated from escherichia coli. It cuts the sequence GAATTC between g and a thus generating 5' sticky ends.


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Scientific paper on DNA replication & gel electrophoresis

... the minimal protein required to sustain oriC and replication were used. Topoisomerase was not used to stall replication after a few minutes -Used Eco RI to cut the DNA -Mapped the bp lengths of the newly replicated strand every 1 minute for 6 minutes. Strands that migrated to the top of the gel ...

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by dekk1525
Sat Sep 08, 2012 11:17 pm
 
Forum: Molecular Biology
Topic: Scientific paper on DNA replication & gel electrophoresis
Replies: 0
Views: 4705

Re: need help with subcloning !!

... 5-6 clones positive out of 24 , in the previous one none ! but I am wondering if my plasmid can self_ligate if it has been double digested ! (bam/eco) why do i have so low positive clones ? and if the eco digestion dint work properly, how do i still have the fragement inside teh strange vector ...

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by Jasmine0507
Wed Dec 21, 2011 4:55 pm
 
Forum: Molecular Biology
Topic: need help with subcloning !!
Replies: 3
Views: 1828

need help with subcloning !!

Hi I am trying to clone a 350bp fragment from a pbabe into a plenti6 with N_terminal flag ha ( 6500bp). For this, I cut out the fragment with BamHI/EcoRI and I digest the plenti with BamHI/EcoRI as well. I obtain the fragment on gel and extract the band using machery_nagel gel exctraction kit, purify ...

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by Jasmine0507
Wed Dec 21, 2011 1:20 pm
 
Forum: Molecular Biology
Topic: need help with subcloning !!
Replies: 3
Views: 1828

Re: electroporation

http://en.wikipedia.org/wiki/Bulgaria Yes even manipulation with bacteria. :( The "eco organisation" provides a lot of pressure on the government. :( And the government ban GMO. :evil:

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by ZEGSUS
Sun Nov 06, 2011 11:08 am
 
Forum: Genetics
Topic: electroporation
Replies: 46
Views: 11893

Photosynthesis Experiment - Do LED's work?

LED lamps work, and they are pretty eco if you ask me (they use less power). But as guys before me said, you need the right colors. Here is my handmade LED lamp: http://img585.imageshack.us/img585/7144/63132836.th.jpg The ...

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by Vaucheria
Thu Jul 07, 2011 3:49 am
 
Forum: Botany Discussion
Topic: Photosynthesis Experiment - Do LED's work?
Replies: 8
Views: 6507
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