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Dyes

Dyes

Coloured chemical substances that impart more or less permanent colour to other materials. They are used for staining and colouring, as test reagents, and as therapeutic agents in medicine.


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Re: centrifugation of hemocytes

... the viability worked well. However, that time i skiped the washing step (and wait 24h...), which can be annoying when working with different dyes... I will try to wash gently and gently take off the fresh-washing-PBS before really resuspending and i'll see... I guess the pellet can't be viewed ...

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by Thom
Tue Jan 28, 2014 4:16 pm
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 2383

hybridization probe

I will use lightcycler 2.0. to detect and SNP with hybridization probes. I designed the probes but I couldn't choose fluorescent dyes, and don't know the criterias. Could you please help me...

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by FDHeLiX
Mon Sep 16, 2013 3:33 pm
 
Forum: Genetics
Topic: hybridization probe
Replies: 2
Views: 1450

Protein ladder width increases down the gel?

... used to indicate the molecular weight of proteins when running a SDS-PAGE. I have conjugated several different molecular weight proteins with dyes and ran them on a 12% gel. There is no problem with the size when I loaded each protein into a separate well, but when I combined all of the proteins ...

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by albert23
Wed Feb 27, 2013 12:55 am
 
Forum: General Discussion
Topic: Protein ladder width increases down the gel?
Replies: 2
Views: 1679

isolation of plasmid (Midi prep)

... is in your plasmid. Do PCR reaction. The second possibility is that the band size does not match theroretical due to electrophoresis problem. Some dyes like sybr green alter dna mobility depending on concentration/concentration manner. Try to run your gel without any fluorecent dye and stain it ...

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by triton
Sat Aug 25, 2012 8:46 am
 
Forum: Molecular Biology
Topic: isolation of plasmid (Midi prep)
Replies: 3
Views: 5085

Dyes used to stain neutrophils

What are the Dyes used to stain neutrophils of blood?

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by kumar985
Wed Jul 25, 2012 5:31 am
 
Forum: General Discussion
Topic: Dyes used to stain neutrophils
Replies: 0
Views: 1077
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