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DsDNA-RT

Definition

noun

A virus which contains a double stranded DNA genome and replicates using a single stranded RNA intermediate.


Supplement

This is a small group of viruses belonging to the Class VII of the Baltimore classification system. An example the Hepadnaviridae family where hepatitis B virus belongs.


Compare: dsDNA virus, dsRNA virus.


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Cell and Molecular Revision Question Help!!! (2)

... for ligations, how much insert would be added to the 50ng of EcoRI digested pBR328 to ensure the best possible chance of successful ligation? (MW dsDNA = 660; MW ssDNA = 330) Again would appreciate some feedback/walkthrough of these questions as I'm currently doing practice questions. Cheers, ...

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by Didact1
Mon Sep 30, 2013 11:35 am
 
Forum: Cell Biology
Topic: Cell and Molecular Revision Question Help!!! (2)
Replies: 0
Views: 1934

Cell and Molecular Revision Question Help!!!

... for ligations, how much insert would be added to the 50ng of EcoRI digested pBR328 to ensure the best possible chance of successful ligation? (MW dsDNA = 660; MW ssDNA = 330) Again would appreciate some feedback/walkthrough of these questions as I'm currently doing practice questions. Cheers, ...

See entire post
by Didact1
Mon Sep 30, 2013 11:34 am
 
Forum: Molecular Biology
Topic: Cell and Molecular Revision Question Help!!!
Replies: 4
Views: 2692

MOLECULAR CLONING

... will be taken up by competent cells and colonies will grow. Any comment on this? Will those nick be gone and will bacteria amplify intact circular dsDNA plasmids? tx

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by kk
Fri Jul 19, 2013 12:50 pm
 
Forum: Molecular Biology
Topic: MOLECULAR CLONING
Replies: 9
Views: 5418

Inserting cDNA into a retroviral vector?..

you will hardly insert dsDNA into ssRNA. You have to make RNA from it and that use for the insertion. But you have to be aware whether your retrovirus contains + or - RNA strand.

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by JackBean
Mon Mar 11, 2013 12:20 am
 
Forum: Genetics
Topic: Inserting cDNA into a retroviral vector?..
Replies: 3
Views: 2633

Mastermix in PCR

... rate at which an incorrect dNTP is incorporated into the resulting PCR product as compared with the original sequence. MgCl2 also can stabilize dsDNA structures. Manipulation of MgCl2 concentration (increasing it) is often used to increase amplification of "hard to amplify" DNA samples. ...

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by daddyjames
Sun Oct 14, 2012 3:43 pm
 
Forum: Bioinformatics
Topic: Mastermix in PCR
Replies: 4
Views: 3387
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