Dropper

... where you don't use a selectively permeable membrane: You have a glass of water and you place a coloured dye to one corner of the glass via eye dropper, then after some dye has been transported into the water, you can see the diffusion of the dye moving from HIGH CONCENTRATION to LOW CONCENTRATION ...

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... where you don't use a selectively permeable membrane: You have a glass of water and you place a coloured dye to one corner of the glass via eye dropper, then after some dye has been transported into the water, you can see the diffusion of the dye moving from HIGH CONCENTRATION to LOW CONCENTRATION ...

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Sounds like insufficient drying and/or over-rinsing. Over-rinsing is a very common problem with new students. Just a few drops from an eye-dropper is usually sufficient for rinse - much more and you end up washing away much of the bacteria. Plus, if the smear was insufficiently dried or you ...

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... to take supernatant use another centrifugation at 5000g for 10 min in cold room. Then I loaded 2mL of the lysate to 3mL 35%sucrose cushion with dropper. I found that some drops distrubed the sucrose layer surface during the loading process. However, I found that there was still two liquid layers ...

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... to take supernatant use another centrifugation at 5000g for 10 min in cold room. Then I loaded 2mL of the lysate to 3mL 35%sucrose cushion with dropper. I found that some drops distrubed the sucrose layer surface during the loading process. However, I found that there was still two liquid layers ...

See entire post