Dictionary » D » Downstream



1. (Science: molecular biology) portions of dna or rna that are more remote from the initiation sites and that will therefore be translated or transcribed later.

2. Shorthand term for things that happen at a late stage in a sequence of reactions.

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Bioinformatics Hands-On NGS Data Analysis Workshops 2014

... microRNA Analysis Using High-Throughput Sequencing will cover the essential steps of small RNA-seq analysis from microRNA prediction to extensive downstream analyses. We also have a workshop on DNA Methylation Analysis where Helene Kretzschmer will show you how she took care of her data in the ...

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by ecSeq
Mon Jul 07, 2014 2:54 pm
Forum: General Discussion
Topic: Bioinformatics Hands-On NGS Data Analysis Workshops 2014
Replies: 0
Views: 956

Re: Muscular dystrophy inheritance question

... puzzled by the top trace. Is it possible that this is representing the sequence of cDNA across a splice site that is spliced alternatively, where downstream of the AAAAAAA the sequence is spliced to different sequences depending on the allele? Why, though, would the male show alternative splicing ...

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by jonmoulton
Thu Jan 10, 2013 3:59 pm
Forum: Molecular Biology
Topic: Muscular dystrophy inheritance question
Replies: 7
Views: 5495

Re: Markerless mutants using suicide vector

... (it couldn't be reproduced!). However, our linear KO DNA also contain an antibiotic cassette that we suspect may introduce some polar effect on downstream genes, so we are trying to avoid that route. If we try to, for example, use linear DNA without some selectable marker, we'll just have a ...

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by dhkwak1
Wed Dec 19, 2012 1:04 am
Forum: Genetics
Topic: Markerless mutants using suicide vector
Replies: 2
Views: 2669

Re: How do we insert a polylinker in lacZ without inactivating i

Likely the polylinker adds a number of bases evenly divisible by three, so it does not frameshift the sequence downstream. You end up with a few extra amino acids added in just after the start of translation. When you clone into the polylinker, you may or may not have a frameshifting ...

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by jonmoulton
Mon Jun 04, 2012 11:22 pm
Forum: Genetics
Topic: How do we insert a polylinker in lacZ without inactivating i
Replies: 2
Views: 1803

what is polycombrepress region ?

... of several Polycomb group (PcG) proteins is deregulated in human cancer suggesting an important role for PcGs in tumor development. Whereas the downstream functions of PcGs are well characterized, the mechanisms of their recruitment to target genes and the regulation of their activity are not ...

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by canalon
Thu Mar 15, 2012 1:22 pm
Forum: Molecular Biology
Topic: what is polycombrepress region ?
Replies: 1
Views: 987
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