Downstream

... intron is included, you are likely to have a stop codon in-frame in the intron. This triggers nonsense-mediated decay of the mRNA. Frameshifting downstream sequence by including an intron with a number of bases not evenly divisible by three will also likely bring a stop codon in-frame in the ...

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... additional nucleotide sequence at the 3’ end of the cDNA. This part codes for an extra 123 amino acids. Annotated gene UB2, appears immediately downstream of YFG. UB2 has a single exon coding for 123 amino acids matching the cDNA. There is no cDNA in your database encoding for a UB2 gene with ...

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... It would appear that we are taking the same module. I came online to look for solutions and stumbled on your post. How does appearing immediately downstream justify it not being a separate stream? in any case, downstream is 5' to 3' i'm quite sure. may I add that this module has turned me off ...

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... additional nucleotide sequence at the 3’ end of the cDNA. This part codes for an extra 123 amino acids. Annotated gene UB2, appears immediately downstream of YFG. UB2 has a single exon coding for 123 amino acids matching the cDNA. There is no cDNA in your database encoding for a UB2 gene with ...

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... is not always true. sometimes an ORF(open reading frame) lacks a ribosome binding site and is only actively transcribed by virtue of the being downstream of a gene with a strong RBS. in these cases, the start codon of the downstream ORF often overlaps the 3' end of the upstream ORF(most often ...

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