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Dot blot

Dot blot

(Science: molecular biology, procedure) method for detecting a specific protein or message. A spot of solution is dotted onto nitrocellulose paper, a specific antibody or probe is allowed to bind and the presence of bound antibody/probe then shown by using a peroxidase coupled second antibody, as in western blotting or by other visualisation methods.


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dot blot

Does any body have any experience with ''dot blot''? http://www.bio-rad.com/prd/en/US/LSR/PDP/d986e28f-4a82-4c13-8ce1-3eae9bb4fdbb/Bio-Dot-and-Bio-Dot-SF-Microfiltration-Apparatus ...

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by Bio2013
Thu Mar 14, 2013 6:07 am
 
Forum: Molecular Biology
Topic: dot blot
Replies: 3
Views: 1501

dot blot--protoplasts

A question regarding protoplasts in a dot blot..my protein of interest is in the protoplast which I has isolated and I have been told to do a titration/dilution ...

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by biology_06er
Wed Jan 11, 2012 1:35 am
 
Forum: Molecular Biology
Topic: dot blot--protoplasts
Replies: 1
Views: 651

How to draw a blot from a RFLP physical map?

... individuals with EcoRI, separate fragments by electrophoresis, blot to a membrane, and hybridize the blot to a labeled probe. Draw a picture ... I am unable to figure out how i can represent a physical map on a DOT blot kindly help me out i really don't understand how it looks like on ...

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by ssimon10
Wed Nov 23, 2011 2:43 pm
 
Forum: Genetics
Topic: How to draw a blot from a RFLP physical map?
Replies: 7
Views: 3005

Re: How to draw a DOT blot from a physical map of RFLP analysis?

=================== (probe) _____|<-1.5kb->|<--2kb-->|<-1kb->|<----4Kb---->| SITE: 1 2 3 4 5 this is the one properly aligned... the probe is on sites 2,3,4 the distance between site 1 and 2 is 1.5kb site 2 and 3 is 2 kb sites 3 and 4 is 1kb and sites 4 and 5 is 4 kb ....i do...

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by supriabio
Fri Nov 27, 2009 7:08 pm
 
Forum: Genetics
Topic: How to draw a DOT blot from a physical map of RFLP analysis?
Replies: 3
Views: 2955

Re: How to draw a DOT blot from a physical map of RFLP analysis?

Bands you can see are those that bound radioactive probe.The way you typed..probe can bind to region 1 and 2.. When you cut with EcoRI DNA from individual A you get two visible bands (with probe) 1.5kb and 2kb..and 2 invisible 1kb and 4kb When you cut DNA from individual B you get again two visible ...

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by zami'87.
Fri Nov 27, 2009 10:01 am
 
Forum: Genetics
Topic: How to draw a DOT blot from a physical map of RFLP analysis?
Replies: 3
Views: 2955
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