Dictionary » D » Dodecyl

Dodecyl

Dodecyl

The radical of dodecane.

dodecyl gallate, dodecyl 3,4,5-trihydroxybenzoate;an antioxidant.


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Western Blot

... prepared from a cell, detergents can be used to make the proteins unfold into the linear chain of amino acids. Using a detergent such as sodium dodecyl sulfate (SDS) makes proteins unfold and gives them a net negative charge that is directly related to their molecular size. Using this feature, ...

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by MarkHolland
Sat Mar 30, 2013 3:22 pm
 
Forum: Cell Biology
Topic: Western Blot
Replies: 6
Views: 3182

Horseradish Peroxidase protein does not SHow up on PAGE gel

... the HRP is SOdium phosphate monohydrate and it worked out alright. I loaded the wells with beta-mercaptoethanol and all the proteins had sodium dodecyl sulphate (SDS), all my proteins ran succesfuly on the gel except HRP which was NON-EXISTENT after i destained all the coomasie blue. Are there ...

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by AlaRoshdieh
Thu May 20, 2010 2:50 am
 
Forum: Molecular Biology
Topic: Horseradish Peroxidase protein does not SHow up on PAGE gel
Replies: 2
Views: 1920

The Fiber Disease

... not found in a similar fraction from uninfected brain. The protein migrated with an apparent molecular size of 27,000 to 30,000 daltons in sodium dodecyl sulfate polyacrylamide gels. The resistance of this protein to digestion by proteinase K distinguished it from proteins of similar molecular ...

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by London
Mon Sep 11, 2006 10:27 pm
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 5414689

ration of q/ms and SDS PAGE

In SDS PAGE we use sodium dodecyl sufate to make the ration of q/m s of two diff peptides nearly equal still keeping the difference between them the same. This tells that this ration is only important here... why??? I think the difference ...

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by 2810712
Tue Jan 17, 2006 5:55 am
 
Forum: Molecular Biology
Topic: ration of q/ms and SDS PAGE
Replies: 0
Views: 838

Paraormaldehyde action in immunofluorescence.

... with aldehydes require a permeabilization step with a detergent. Triton X-100 is a popular permeabilizing agent, as are saponin, Tween, and sodium dodecyl sulfate (SDS). After permeabilization, cells are treated with a primary antibody (or a mixture of two different antibodies from different hosts) ...

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by chemistry_freako
Tue Jul 26, 2005 4:36 am
 
Forum: Cell Biology
Topic: Paraormaldehyde action in immunofluorescence.
Replies: 8
Views: 9250


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