Dnase i

Have you even checked the gram stain?! If you did, I may be able to tell you off the top of my head. How can you be working on identification and not have a manual? It doesn't have to be the one you mentioned, other people have published manuals too. Try a library.

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Of these four bacteria are any positive for for DNase? I've searched and searched and cannot find (reliable) information on this. I do not have access to Bergey's manual. Pseudomonas aeruginosa, Pseudomonas fluorescens, Proteus mirabilis, and Citrobacter ...

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... I guess the only tricky part will be the preparation of DNA. To clone it, I will need manageable sizes of DNA so I might have to treat it with DNAse or nucleases. How much should I add? For the electrophoretic mobility shift assay, how would I detect it? Maybe a Western blot?

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Put the gene under the control of an inducible promoter. That way your introduced DNAse can be selectively expressed.

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Hi all, For a project I'm currently working on I need to transfect a disease cell line with DNASE 1 to investigate its interaction. The problem is this molecule has a role in cell apoptosis. Is there a way I can transfect my cell lines without nuclear DNA destruction? ...

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