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Dna polymerase

Dna polymerase

(Science: enzyme molecular biology) enzymes involved in template directed synthesis of dna from deoxyribonucleotide triphosphates.


i, II and III are known in E. Coli, III appears to be most important in genome replication and i is important for its ability to edit out unpaired bases at the end of growing strands. Animal cells have and polymerases, with apparently responsible for replication of nuclear dna and for replication of mitochondrial. All these function with a dna strand as template. Retroviruses possess a unique dna polymerase (reverse transcriptase) that uses an rna template.


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Purpose of a primase/polymerase localized to the cytosol?

Hi, I recently read a paper, "PrimPol, an Archaic Primase/Polymerase Operating in Human Cells" by Garcia-Gomez et al. This polymerase was largely localized to the cytosol - I'm wondering if anyone has ideas as to why that would be the case or ...

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by rhardison
Fri Oct 03, 2014 8:51 pm
 
Forum: Molecular Biology
Topic: Purpose of a primase/polymerase localized to the cytosol?
Replies: 0
Views: 20

Re: Any ideas for vaccine development or anti-viral drugs???

... morpholino oligomers (PMO) are a class of uncharged single-stranded DNA analogs modified such that each subunit includes a phosphorodiamidate ... of viral mRNAs for the viral proteins (VPs) VP24, VP35, and RNA polymerase L protected rodents in both pre- and post-exposure therapeutic ...

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by jonmoulton
Mon Sep 15, 2014 3:04 pm
 
Forum: Cell Biology
Topic: Any ideas for vaccine development or anti-viral drugs???
Replies: 2
Views: 476

Annealing Temparature

... at 50°C and the rest at 65°C. Denaturation for 10s will be enough, but a bit higher temperature 95-98°C might be better. Elongation depends on polymerase, so 1 min can be enough for Phusion, but 4 min will be required for Pfu.

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by vladCH
Sat Feb 22, 2014 7:54 pm
 
Forum: Molecular Biology
Topic: Annealing Temparature
Replies: 4
Views: 1577

Annealing Temparature

... in the beginning is only 1 min or is it a typo? Why do you have such long denaturation during cycling? Why do you have such long annealing? What polymerase do you use? What is the GC content of your GOI? How many cycles did you have? What is the template? What concentration did you use?

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by JackBean
Tue Feb 18, 2014 12:40 pm
 
Forum: Molecular Biology
Topic: Annealing Temparature
Replies: 4
Views: 1577

A question regarding PCR

probably, your microsatellite is part of the chromosome, right? So there will be much more than 7 bp and it will be on both sides. Yes, the polymerase will amplify the chain until the conditions change (i.e. end of amplification step) or until it falls off (which may happen with very long amplicons, ...

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by JackBean
Sat Jan 18, 2014 2:36 pm
 
Forum: Molecular Biology
Topic: A question regarding PCR
Replies: 1
Views: 1458
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