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Dna methylation

Dna methylation

(Science: molecular biology) process by which methyl groups are added to certain nucleotides in genomic dna.

this affects gene Expression, as methylated dna is not easily transcribed. The degree of methylation is passed on to daughter strands at mitosis by maintainance dna methylases. Accordingly, dna methylation is thought to play an important developmental role in sequentially restricting the transcribable genes available to distinct cell lineages.

in bacteria, methylation plays an important role in the restriction systems, as restriction enzymes cannot cut sequences with certain specific methylations.


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Re: Sau3A problem....

I would start over with fresh DNA and fresh enzymes. The weakest link in the system is ligase. If you're satisfied ... some way. I don't know much about Pseudomonas . I assume there are no issues with methylation.

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by blcr11
Thu Sep 18, 2008 1:54 pm
 
Forum: Molecular Biology
Topic: Sau3A problem....
Replies: 2
Views: 820

Re: IBO 2003 questions- please help me out

... growth hormone. It is a peptide hormone made in the pituitary gland. DNA polymerase comes in a variety of flavors, but the major eukaryotic DNA ... DNA into nucleosomes and may be important regulators of cytosine methylation. The function of seed storage proteins is to act as a deterrant ...

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by blcr11
Mon May 12, 2008 5:54 pm
 
Forum: Cell Biology
Topic: IBO 2003 questions- please help me out
Replies: 9
Views: 1371

Gene methylation

What does methylating a sequence of DNA do to the DNA?

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by B10Student
Thu May 08, 2008 5:28 am
 
Forum: Molecular Biology
Topic: Gene methylation
Replies: 2
Views: 725

Functional relationship between Proteins and DNA

... (1) (2) DMS Footprinting Genetic analysis X-ray crystallography Methylation Interference Mapping protien/DNA interaction by cross-linking Articles from a range of Nature Publishing Group journals ...

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by pguo
Mon May 05, 2008 8:34 am
 
Forum: Molecular Biology
Topic: Functional relationship between Proteins and DNA
Replies: 2
Views: 544

New theory... ?

... quite a lot of things can be transmitted by other means, for example DNA methylation state, presence of some proteins, regulatory RNA etc...

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by canalon
Tue Apr 22, 2008 1:27 pm
 
Forum: Genetics
Topic: New theory... ?
Replies: 5
Views: 880
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