Divalent

... the pH adjusted to 8?" Now as a beginning of an answer: NaCl is used to prevent the co-precipitation of DNA and CTAB. EDTA is used to chelate divalent cations, which are necessary (among other things) for proper DNASe activity Tris is a Buffer CTAB is a cationic detergent that precicpitates ...

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... The technique used to separate according to size is gel filtration chromatography. apain incubated with EDTA: Activates enzyme activity by binding divalent metal cationshave no effect on enzyme activty activate enzyme activty by modifying the casein substrate inhibit enzyme activty by binding divalent ...

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... the light absorption will go off the scale the initial rate is always the slowest Papain incubated with EDTA: Activates enzyme activity by binding divalent metal cations have no effect on enzyme activty activate enzyme activty by modifying the casein substrate inhibit enzyme activty by binding ...

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TE is TRis EDTA. Tris is a buffer, EDTA is an chelator for divalent cations. Since many DNAse use metallic cations (Mg2+ etc..) as coenzyme, if they are chelated, they will not be able to degrade your DNA during extraction.

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thank you..but i worry that the EDTA (in tris) will cause problems later to my pcr as it chelates the divalent cations of Mg+2...but when i use depc H2O i have a difficulty in diluting my dna as it is acidic and the dna is diluting in slightly alcalic pH...THANK YOU FOR THE ...

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