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Disulfide

Disulfide

1. A molecule containing two atoms of sulfur to one of the reference element, e.g., CS2, carbon disulfide.

2. A compound containing the -S-S-group, e.g., cystine.


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question about G418 sceening test

... each is about 15% confluence. After 24hr incubation with 2 mL regular DMEM, I changed to DMEM with G418 with different concentrations. The G418 disulfide 50mg/mL stock in H2O was from Sigma(G8168). I simply added it to 2mL to create a 400,500,600,700,800,900 ug/mL serie. It has been reported ...

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by cavaliersun2003
Mon Nov 11, 2013 7:28 am
 
Forum: Cell Biology
Topic: question about G418 sceening test
Replies: 0
Views: 1029

cellular mechanisms that result in symptoms of cholera

... complex made up of six protein subunits: a single copy of the A subunit (part A), and five copies of the B subunit (part B), connected by a disulfide bond. The five B subunits form a five-membered ring that binds to GM1 gangliosides on the surface of the intestinal epithelium cells. The ...

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by adithya528
Tue Dec 11, 2012 1:49 pm
 
Forum: Cell Biology
Topic: cellular mechanisms that result in symptoms of cholera
Replies: 4
Views: 5043

Re: How to prove that thiol groups are de-protected?

MALDI-TOF mass spec comes to mind to check if the disulfide is broken. I suppose it depends what instruments are at hand.

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by jonmoulton
Wed May 02, 2012 10:10 pm
 
Forum: Molecular Biology
Topic: How to prove that thiol groups are de-protected?
Replies: 2
Views: 1450

Help me pleeeeeeease

That depends on particular enzyme. Some may dimerize after e.g. phosphorylation, glycosylation or other modification, some may dimerize due to disulfide bridge, some may have even other mechanism.

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by JackBean
Wed Apr 18, 2012 8:05 am
 
Forum: Human Biology
Topic: Help me pleeeeeeease
Replies: 3
Views: 1596

2ME and SDS

As canalon said, the program calculates MW of only one subunit, but obviously, they are connected by disulfide bonds to form tetramer.

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by JackBean
Tue Nov 15, 2011 11:25 am
 
Forum: Molecular Biology
Topic: 2ME and SDS
Replies: 2
Views: 1280
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