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Dictionary » D » Disulfide DisulfideDisulfide 1. A molecule containing two atoms of sulfur to one of the reference element, e.g., CS2, carbon disulfide. ![]()
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Results from our forumcellular mechanisms that result in symptoms of cholera... complex made up of six protein subunits: a single copy of the A subunit (part A), and five copies of the B subunit (part B), connected by a disulfide bond. The five B subunits form a five-membered ring that binds to GM1 gangliosides on the surface of the intestinal epithelium cells. The ...
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Re: How to prove that thiol groups are de-protected?MALDI-TOF mass spec comes to mind to check if the disulfide is broken. I suppose it depends what instruments are at hand.
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Help me pleeeeeeeaseThat depends on particular enzyme. Some may dimerize after e.g. phosphorylation, glycosylation or other modification, some may dimerize due to disulfide bridge, some may have even other mechanism.
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2ME and SDSAs canalon said, the program calculates MW of only one subunit, but obviously, they are connected by disulfide bonds to form tetramer.
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2ME and SDS... The computer will just the size of the sequence you fed it and calculate a size based on that. It does not magically analyse the protein to infer disulfide bonds and such.
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