Disintegrations

Hi, thx for the reply.. Well I think i get the explanation but in our experiment we used CP55940 with the radioligand SR141716A in and not in the presence of non-hydrolysable analogue, GTPgammaS..what was seen that when the GTPgS was not present, as the concentration of CP55 decrease the CPM increas...

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Should have said it the other way around: you don't normally plot dpm directly as a function of concentration of ligand. Usually you've transformed the dpm or cpm readout into some form that observes saturation phenomenon, like %B/F or %B.

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The dpm are the disintegrations per minute your detector sees due to the decay of the radioactive label present in the assay tube. From the sounds of it, you are being asked to plot the dpm for a standard curve? If you really need ...

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Hi there, We recently carried out a radioligand binding assay and it says we must graph the DPMs along the y axis with conc. on the x-axis...just wondering what exactly DPMs are..is it the count of how much ligand is bound to the receptor :oops: :oops: Thanks, b_06er

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... River, North Carolina, was chemotactically attracted to amino acids when tested in a radioassay. The bacterium was labeled with P(i), and the disintegrations per minute determined by liquid scintillation counting were proportional to the number of cells accumulating in microcapillaries containing ...

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