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Disc electrophoresis

short for discontinuous electrophoresis, it is a type of polyacrylamide gel electrophoresis. this electrophoresis method uses gels of two different concentrations of polyacrylamide (a synthetic polymer), the one of lower concentration stacked on top of the one with higher concentration, in order to better resolve bands of whatever is being separated (dna, rna, or protein) that would otherwise be very close together.