Dimer

... antibodies to crosslink the corresponding receptors. I do not know the exact reason. 1. Why needs crosslinking using Abs? Does crosslinking make dimer or trimer of receptor formation? 2. What the signaling effects after crosslinking? active or block? 3. Which kind of antibodies can be used to ...

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Looks OK to me, unless the dimer is the relevant molecule.

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... product should be double digested well. I drew my conclusion based on the self-ligation results. The double-digested PCR product was able to form dimer, trimer,tetramer,even pentamer. If only one enzyme worked, only monomer and dimer would be observed. (2). The ligation reaction, the total volume ...

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... and long-time digestion. (2). Self ligation control vector: ladder has been observed insert: ladder has been observed, which includes monomer, dimer, trimer...This implicates both NdeI and SmaI worked, otherwise I should see only monomer and dimer if only one enzyme worked. (3). Uncut vector ...

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:D Thank you!! You helped me a lot!!!

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