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Dilutions

Dilution

1. The act of being diluted.

2. A diluted solution or mixture.

3. In microbiologic techniques, a method for counting the number of viable cells in a suspension; a sample is diluted to the point where an aliquot, when plated, yields a countable number of separate colonies.


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MolBio calculations

... how much 1 pmol weights (in mass units) ad thus it should be easy to calculate the volume when you know the concentration. That's just a lots of dilutions. In the beginning you have 0.25 ug/100ul = 250 ng/100ul = 2.5 ng/ul. Which is quite useless for you :-D OK, so you have 0.25 ug and you dilute ...

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by JackBean
Wed Dec 04, 2013 8:12 pm
 
Forum: Molecular Biology
Topic: MolBio calculations
Replies: 1
Views: 1653

CFU

... and spread plate experiment, a student counted the following number of colonies on nutrient agar plates inoculated with 100 l of respective dilutions from an original broth culture of E. coli. 10-5 = Too many to count (TMTC) colonies 10-6 = 248 colonies 10-7 = 33 colonies 10-8 = 1 colony ...

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by angel92
Mon Jul 22, 2013 9:42 pm
 
Forum: Molecular Biology
Topic: CFU
Replies: 1
Views: 1080

Effect Of carbon dioxide on rate of photosynthesis

... should be checked to prove that it is indeed oxygen - relights a glowing splint. Repeat at different lower CO2 concentrations by using different dilutions of a saturated solution. Graph the results placing CO2 concentration on the x-axis.

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by snowfall
Thu May 30, 2013 5:33 pm
 
Forum: Botany Discussion
Topic: Effect Of carbon dioxide on rate of photosynthesis
Replies: 4
Views: 6067

What bacteria could this be?

... studying BSc Biology and for part of my food microbiology assessment, we have had to grow bacteria from cheese types in cultures (of different dilutions). So I have managed to identify all them apart from this one, I was wondering if anyone could point me in the right direction. The bacteria ...

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by jessaimeewhite
Sat Dec 08, 2012 1:34 pm
 
Forum: Microbiology
Topic: What bacteria could this be?
Replies: 0
Views: 1433

cell number dilutions

Hi..I jus started wrk in a cancer bio lab wer i amsupposed to do anxtt preassay optimisation with diff cell num 4 thp1 cellline..numbers from 10*10^4..8*10^4....6*10^4...4*10^4....and 2*10^4..how to do do it say aftwer trypan blue hemocytometer count i get 42*10^4 cells/ml..plz help

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by ankitaalive
Wed Nov 28, 2012 6:22 am
 
Forum: General Discussion
Topic: cell number dilutions
Replies: 1
Views: 693
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