Dilutions

... studying BSc Biology and for part of my food microbiology assessment, we have had to grow bacteria from cheese types in cultures (of different dilutions). So I have managed to identify all them apart from this one, I was wondering if anyone could point me in the right direction. The bacteria ...

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Hi..I jus started wrk in a cancer bio lab wer i amsupposed to do anxtt preassay optimisation with diff cell num 4 thp1 cellline..numbers from 10*10^4..8*10^4....6*10^4...4*10^4....and 2*10^4..how to do do it say aftwer trypan blue hemocytometer count i get 42*10^4 cells/ml..plz help

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With the fluorometric enzyme Assay how to convert the FLU/min (obtained as FlU/min slope for every concentration of substrate; serial dilutions of substrate at concentrations 200 uM to 50 uM), into to RFU/mg/min of enzyme, that is nessesary to obtain the rate of reaction for the enzyme. Enz. ...

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... as good as Qubit (different measurements) but I believe Qubit is more accurate. By the way how do you prepare your std curve exactly? How many dilutions do you make? What kin of cycler do you use?

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... the absorbance spectra of potential substrates and products as the following: OAA stock is 8 mM Malate stock is 8 mM NADH stock is 1 mM we made dilutions of these with water as shown below: Dilution of 1:2 OAA: 500 μL water + 500 μL OAA malate: 500 μL water + 500 μL malate NADH: 500 μL water ...

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