Dictionary » D » Dilution

Dilution

Dilution

1. The act of being diluted.

2. A diluted solution or mixture.

3. In microbiologic techniques, a method for counting the number of viable cells in a suspension; a sample is diluted to the point where an aliquot, when plated, yields a countable number of separate colonies.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Questions about DNA dialysis

... becomes the final volume, and the salts are diluted proportionately. Like, V1/V2 * inititial salt conc. If you want to, rather than calculate dilution, you can just dialyze in large volume, or do several sequential dialysese (how do you pluralize?) to get close to nothing, then add salts/ingredients ...

See entire post
by biomart
Wed Mar 11, 2015 7:43 am
 
Forum: Bioinformatics
Topic: Questions about DNA dialysis
Replies: 1
Views: 311

Questions about DNA dialysis

... of dialysis. Can you give me some literature hints, please? Secondly, I know my target DNA concentration, but I know dialysis can lead to dilution and sample loss. How much higher shall I choose the starting concentration? Also, how can I calculate the optimum time for dialysis, the right ...

See entire post
by protoemics
Thu Feb 05, 2015 7:56 am
 
Forum: Bioinformatics
Topic: Questions about DNA dialysis
Replies: 1
Views: 311

How would I do this experiment? [Advanced]

As you must be able to grow the bacteria (otherwise you cannot perform the test at all), it would be better to perform dilution of your samples and spread aliquots of such on plates with growth agar (for E.coli simple LB, for Kefir, no idea). Let them growth (E.coli for 1 day at 37°C) ...

See entire post
by JackBean
Fri Dec 19, 2014 2:46 pm
 
Forum: Cell Biology
Topic: How would I do this experiment? [Advanced]
Replies: 2
Views: 1059

Re: How would I do this experiment? [Advanced]

... to count them separately? If you find a selective stain for E.coli you may be able to count them separately. Also, what you can do is, use serial dilution technique and then pick up colonies on final plates and distinguish as Kefir or E. coli based on microscopic observations and possibly some ...

See entire post
by Hrushikesh
Mon Dec 15, 2014 5:00 pm
 
Forum: Cell Biology
Topic: How would I do this experiment? [Advanced]
Replies: 2
Views: 1059

Re: A dilution question about RNA

Why, for God's sake would you make a DNase treatment in 1ml??? :shock: You would take around 30 ul of your RNA (100ug / 3.3ug/ul), add the DNase worth few tens of bucks at least, 100 ul of 10x buffer and water up to 1 ml.

See entire post
by JackBean
Thu Feb 06, 2014 4:31 pm
 
Forum: Molecular Biology
Topic: A dilution question about RNA
Replies: 1
Views: 1576
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 7,991 times. 
What links here | Related changes | Permanent link