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Dilution

Dilution

1. The act of being diluted.

2. A diluted solution or mixture.

3. In microbiologic techniques, a method for counting the number of viable cells in a suspension; a sample is diluted to the point where an aliquot, when plated, yields a countable number of separate colonies.


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Re: A dilution question about RNA

Why, for God's sake would you make a DNase treatment in 1ml??? :shock: You would take around 30 ul of your RNA (100ug / 3.3ug/ul), add the DNase worth few tens of bucks at least, 100 ul of 10x buffer and water up to 1 ml.

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by JackBean
Thu Feb 06, 2014 4:31 pm
 
Forum: Molecular Biology
Topic: A dilution question about RNA
Replies: 1
Views: 770

A dilution question about RNA

... I would like to dilute my RNA to 100 ug and use 100 unite enzyme in 1000 uL reaction mix. Is this logic true and if it is how I am going to do the dilution? Thanks!

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by golgicik
Mon Feb 03, 2014 6:30 pm
 
Forum: Molecular Biology
Topic: A dilution question about RNA
Replies: 1
Views: 770

CFU

... type the number you got: 10^-6 - 248 CFU/100ul 10^-7 - 33 CFU/100ul 10^-8 - 1 CFU/100ul 2. just ten times more obviously 3. just divide CFU/ml by dilution (e.g. 10/10^-8 = 1×10^9), so you'll get 2.48×10^9; 3.3×10^9; 1×10^9 (although I would take the last one out) and make average

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by JackBean
Mon Jul 29, 2013 10:48 am
 
Forum: Molecular Biology
Topic: CFU
Replies: 1
Views: 1143

CFU

Following a serial dilution and spread plate experiment, a student counted the following number of colonies on nutrient agar plates inoculated with 100 l of respective dilutions from an original broth culture of E. coli. 10-5 = Too ...

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by angel92
Mon Jul 22, 2013 9:42 pm
 
Forum: Molecular Biology
Topic: CFU
Replies: 1
Views: 1143

Re: Bacterial Calculation

Hallo, You don`t need a serial dilution calculator ... seriously. If you get 55 colonies at a 10^4 dilution, then you have 5.5*10^5 cfu/mL (in case you used an aliquot of 1 mL). If you count 85 colonies at a 10^5 dilution then it`s 8.5*10^6 ...

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by EnricoPallazzo
Sun Jan 13, 2013 9:43 am
 
Forum: Microbiology
Topic: Bacterial Calculation
Replies: 3
Views: 3153
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