Dilution

hi every body does any body did limiting dilution for creating single cell clones? is it a straightforward way or not? any help or protocol will be appreciated best

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Also, would I use a dilution factor when doing a cell count for a concentrated sample?

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... I am working on calculations for this same lab. The standard I started with was created from 10mL of culture plus 1mL of TCA, so it has a dilution factor of 1.1 I then created a diluted sample from this standard by removing 0.5mL from it, and adding 0.5mL of water to it. Should I take ...

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... in order to overcome some of those difficulties. In my case I use MoBio powersoil and DNA that contained quite some inhibitors, even at high dilution (I am doing qPCR and is very easy to see inhibitors in thse conditions) amplified without problem with this kit. I think epicentre (who also ...

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... So you dilute the samples to reduce the amount of glucose in the assay to get better accuracy. You then have to adjust the assay value by the dilution factor to calculate the glucose level in the original, undiluted plasma sample. Say you diluted the sample by a factor of 10 (1 part plasma ...

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