Diluted

... this same lab. The standard I started with was created from 10mL of culture plus 1mL of TCA, so it has a dilution factor of 1.1 I then created a diluted sample from this standard by removing 0.5mL from it, and adding 0.5mL of water to it. Should I take into consideration the dilution factor of ...

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... will be harder". :roll: ??? Where ARE these s***ty textbooks, I've NEVER seen this. This is nonsense. The FACT is, if your stomach acid is diluted by food or drink, IT JUST MAKES MORE. Your stomach is not a passive beaker in chemistry class - it is a living organ and it adjusts to its environment.

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... Patient plasma samples were prepared by adding 0.3ml of plasma to 2ml of a suitable buffer and then adding a further 1ml of a solution to the diluted plasma to stabilise it. Assay mixtures were prepared in a final volume of 3ml and contained 0.5ml of the stabilised diluted plasma, 0.15mM NADH ...

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I recall diluted media were better for pigment production by Chromobacterium violaceum. Try R2A or 1/2 1/4 strength NA.

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... the assay to get better accuracy. You then have to adjust the assay value by the dilution factor to calculate the glucose level in the original, undiluted plasma sample. Say you diluted the sample by a factor of 10 (1 part plasma + 9 parts distilled water) and determine the glucose concentration ...

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