Dictionary » D » Diluted


Reduced in strength or concentration or quality or purity; diluted alcohol; a dilute solution; dilute acetic acid.

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Questions about DNA dialysis

... It should work by simply allowing the ions to diffuse across the membrane so that the initial volume becomes the final volume, and the salts are diluted proportionately. Like, V1/V2 * inititial salt conc. If you want to, rather than calculate dilution, you can just dialyze in large volume, or ...

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by biomart
Wed Mar 11, 2015 7:43 am
Forum: Bioinformatics
Topic: Questions about DNA dialysis
Replies: 1
Views: 287

Two-color in situ help

Problem seems to be solved. Not sure which step went wrong but the second signal began to appear when I diluted the second antibody 2X more and used less incubation time for the TSA reaction (about 3 min).

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by vastgenome
Sat Mar 09, 2013 4:21 pm
Forum: Molecular Biology
Topic: Two-color in situ help
Replies: 3
Views: 2938

BAP, brassinolide, triacontanol, Amino, kelp foliar question

... time and cost all come together when it calls for testing species by species and PGR by PGR. :x I have a bottle of 5% Gibberellin in liquid form. Diluted to 100pmm to 500ppm and foliar sprayed on Stevia raubadiana, Salvia officinalis, Orchid (I do not know the species), and few other plants. They ...

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by Maton
Sun Dec 23, 2012 2:59 pm
Forum: Botany Discussion
Topic: BAP, brassinolide, triacontanol, Amino, kelp foliar question
Replies: 15
Views: 28155

Re: separation problem with GelRed

I got the tip from another lab to dilute the DNA ladder and now it looks so much better, nice separation. We have diluted the ladder 1:10. Good for the lab-economy as well! Maybe one could go further even, and also dilute the markers- have not tried this yet. When I looked around ...

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by annakarinring
Tue Oct 30, 2012 12:50 pm
Forum: Molecular Biology
Topic: separation problem with GelRed
Replies: 12
Views: 19576

Multiple reactions buffer and enzymatic special activity

... 1 mM MgCl2 and 2 mg/ml bovine serum albumin? Can you please explain in details??? How the buffer was made in detail initially and then how it was diluted from 10X to 1X.

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by dalin
Sun Oct 21, 2012 5:29 am
Forum: Molecular Biology
Topic: Multiple reactions buffer and enzymatic special activity
Replies: 7
Views: 3779
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