Dictionary » D » Dilute

Dilute

Dilute

1. To make thinner or more liquid by admixture with something; to thin and dissolve by mixing. Mix their watery store. With the chyle's current, and dilute it more. (Blackmore)

2. To diminish the strength, flavor, colour, etc, of, by mixing; to reduce, especially by the addition of water; to temper; to attenuate; to weaken. Lest these colours should be diluted and weakened by the mixture of any adventitious light. (Sir i. Newton)

Origin: L. Dilutus, p. P. Of diluere to wash away, dilute; di- = dis- _ luere, equiv. To lavare to wash, lave. See Lave, and cf. Deluge. Lessen the strength or flavor of a solution or mixture; cut bourbon.Reduced in strength or concentration or quality or purity; diluted alcohol; a dilute solution; dilute acetic acid.A substance with 'watered down chemical properties by adding a chemical that can neutralise its effect.


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A dilution question about RNA

... 3313 ng/uL (Around 165 ug). Now my next step is DNase treatment. It says 1 unit of enzyme for 1 ug of RNA in 10 ul reaction mix. I would like to dilute my RNA to 100 ug and use 100 unite enzyme in 1000 uL reaction mix. Is this logic true and if it is how I am going to do the dilution? Thanks!

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by golgicik
Mon Feb 03, 2014 6:30 pm
 
Forum: Molecular Biology
Topic: A dilution question about RNA
Replies: 1
Views: 769

MolBio calculations

... dilutions. In the beginning you have 0.25 ug/100ul = 250 ng/100ul = 2.5 ng/ul. Which is quite useless for you :-D OK, so you have 0.25 ug and you dilute it as follows: 100 ul to fin. volume 500 ul (i.e. 5×). That added to fin. volume 5 ml (i.e. 10×). From that you take 50 ul, which is 1/100th ...

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by JackBean
Wed Dec 04, 2013 8:12 pm
 
Forum: Molecular Biology
Topic: MolBio calculations
Replies: 1
Views: 1797

Re: Urea and membrane permeability of RBC

... the cell until the osmolarity is the same inside and outside the cell. The cell will swell and lyse. If you put the red blood cell into a more dilute urea solution, say one that has half the number of osmoles as normal solution, about 150 mosmole/L, water will immediately start going into the ...

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by kidprof
Thu Aug 08, 2013 8:18 pm
 
Forum: Cell Biology
Topic: Urea and membrane permeability of RBC
Replies: 2
Views: 33893

Problem with the normalization of PCR Ct values

... about the ct values, the higher the value, less copies are there, if you make a standard curve you can see how the ct values get higher when you dilute your DNA more. about the formulas of ct value differences, you should check the help section of the software you are using of the cycler's machine, ...

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by bravebeaker
Wed May 01, 2013 1:12 am
 
Forum: Molecular Biology
Topic: Problem with the normalization of PCR Ct values
Replies: 5
Views: 3667

Protein ladder width increases down the gel?

... solution. In addition, I loaded 1X loading buffer dye in the empty wells. Any ideas? Also, I'm afraid that the bands will not be as prominent if I dilute it further. Thank you in advance!

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by albert23
Wed Feb 27, 2013 5:12 pm
 
Forum: General Discussion
Topic: Protein ladder width increases down the gel?
Replies: 2
Views: 1717
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