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Dilute

Dilute

1. To make thinner or more liquid by admixture with something; to thin and dissolve by mixing. Mix their watery store. With the chyle's current, and dilute it more. (Blackmore)

2. To diminish the strength, flavor, colour, etc, of, by mixing; to reduce, especially by the addition of water; to temper; to attenuate; to weaken. Lest these colours should be diluted and weakened by the mixture of any adventitious light. (Sir i. Newton)

Origin: L. Dilutus, p. P. Of diluere to wash away, dilute; di- = dis- _ luere, equiv. To lavare to wash, lave. See Lave, and cf. Deluge. Lessen the strength or flavor of a solution or mixture; cut bourbon.Reduced in strength or concentration or quality or purity; diluted alcohol; a dilute solution; dilute acetic acid.A substance with 'watered down chemical properties by adding a chemical that can neutralise its effect.


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Protein ladder width increases down the gel?

... solution. In addition, I loaded 1X loading buffer dye in the empty wells. Any ideas? Also, I'm afraid that the bands will not be as prominent if I dilute it further. Thank you in advance!

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by albert23
Wed Feb 27, 2013 5:12 pm
 
Forum: General Discussion
Topic: Protein ladder width increases down the gel?
Replies: 2
Views: 157

Protein ladder width increases down the gel?

these problems usually arise when you have something very different in neighboring wells. If possible, try to dilute it in some buffer and use the same buffer in surrounding wells and you'll see.

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by JackBean
Wed Feb 27, 2013 10:48 am
 
Forum: General Discussion
Topic: Protein ladder width increases down the gel?
Replies: 2
Views: 157

Problems making chemically competent cells

... (30mM) 3ml (1M stock pH 7.5, 9.814g in 100ml solution, pH with acetic acid) CaCl2•2H2O (10mM) 0.15g Glycerol (15% w/v) 15g Final pH: 5.8 with dilute acetic acid. Don’t overshoot – if you do, start again RF2 Compound (end concentration) Amount for 100ml RbCl (10mM) 0.12g MOPS (10mM) 2ml (stock ...

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by lilruthc
Fri Feb 08, 2013 1:09 pm
 
Forum: Molecular Biology
Topic: Problems making chemically competent cells
Replies: 1
Views: 353

Re: Bacterial Calculation

... already close to the concentration you need. To get more precise values, you could grow the cells to a higher density than you actually need and dilute them to the desired concentration.

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by EnricoPallazzo
Sun Jan 13, 2013 9:43 am
 
Forum: Microbiology
Topic: Bacterial Calculation
Replies: 3
Views: 682

qPCR

... PCR for standard curve-the standard. I should quantitate the transcript (RNA) before reverse transcribe them into cDNA. I wonder I should serially dilute the transcript and perform reverse transcription? Or I should synthesis the cDNA first and only perform serial dilution for qPCR? Any different? ...

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by xiaolong88
Tue Dec 11, 2012 4:58 pm
 
Forum: Molecular Biology
Topic: qPCR
Replies: 2
Views: 529
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