Diluent

Greetings, I did the following: 1. Blended 25 g of meat with 225 ml of diluent (1:10 dilution) 2. Performed serial dilutions for above(1:100, 1:1000 and 1:10000) 3. Inoculated 1 ml of the solution of PCA (Plate count agar) 3. Counted the aerobic colonies (ACC) ...

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There are some protocols published for that but I thought that for most of this kind of organisms, samples should be 25g and the diluent peptone water (225ml). But that was a long time ago. I think for your homogenate, 1g might be a little too small a sample. For O157:H7 no need for blood ...

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... O157:H7: in lamb meat.I will grow the sample homogenates on Sorbitol MacConkey Agar! For preparing the sample homogenate, what is the preferable diluent to be used? Why ?Is it sterile water, MRD, MacConkey broth, thyioglycolate ?? Is it it enough to prepare it from 1 g of meat?? Thank u for ur ...

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dilution is simply = amt of diluent/total volume 2/6 <--- this is the dilution 6/2 <---- dilution factor Thus dilution factor = total volume / original volume before dilution ;)

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dilution is simply = amt of diluent/total volume 2/6 <--- this is the dilution 6/2 <---- dilution factor Thus dilution factor = total volume / original volume ;)

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