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Diluent

Diluent

1. That which dilutes.

2. (Science: medicine) An agent used for effecting dilution of the blood; a weak drink. There is no real diluent but water. (Arbuthnot)


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Re: Dilution problem for my Bradfords assay

... standards I used to construct the absorbance/conc curve. I'm just wondering if you can help me figure out how adding 2mg/ml BSA stock with 10ul diluent and 150ul of bradford reagent gives a final BSA conc of 1500ug/ml. I suck at maths and im having a hard time understanding how this was calculated.

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by moabubaker
Fri Oct 19, 2012 12:33 pm
 
Forum: Molecular Biology
Topic: Dilution problem for my Bradfords assay
Replies: 3
Views: 983

Pharmaceutical microbiology - E. coli enumeration

Add some sterile diluent in an appropriate stocmacher bag and use a stomacher to disperse. Plate and quantitate to cfu/gram. There are plenty of methods available - if one is specified by a gov body in your area (like BAM) I'd use ...

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by JorgeLobo
Fri Aug 05, 2011 1:36 pm
 
Forum: Microbiology
Topic: Pharmaceutical microbiology - E. coli enumeration
Replies: 6
Views: 4829

Re: Dilution question

... dilute it down to 2 fiddledeecups, well that's a dilution factor of 18/2 = 9 . So, if I want 9 mL, I use 1 mL of 18 fiddledeecups and add 8 mL of diluent (water in this case). Since the dilution factor is 9, make your final volume a multiple of 9 to make your calculations easier.

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by clevermizo
Thu May 13, 2010 7:54 pm
 
Forum: Molecular Biology
Topic: Dilution question
Replies: 3
Views: 1186

Re: Serial Dilution BSA Bradford measurement

... is 0.15 mg/ml for 1mg/ml I have 15mg/ml x V1 = 1ml x 1mg/ml => V1 = 1/15=0.067ml Thus I take 0.067ml from 10x stock solution and add 0.023ml diluent? I think, that 1 ml - 0.067 ml is not 0.023, but rather like 0.933 ml? The equation is perfect, but you can try to think about it just simply. ...

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by JackBean
Fri May 07, 2010 8:38 pm
 
Forum: Molecular Biology
Topic: Serial Dilution BSA Bradford measurement
Replies: 5
Views: 6652

Re: Serial Dilution BSA Bradford measurement

... to the equation: for 1mg/ml I have 15mg/ml x V1 = 1ml x 1mg/ml => V1 = 1/15=0.067ml Thus I take 0.067ml from 10x stock solution and add 0.023ml diluent? What do you think about that? :?

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by vlad81
Fri May 07, 2010 6:52 pm
 
Forum: Molecular Biology
Topic: Serial Dilution BSA Bradford measurement
Replies: 5
Views: 6652
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