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Diffraction

Diffraction

When a wave train passes an obstacle secondary waves are set up that interfere with the primary wave and give rise to bands of constructive and destructive interference. Around a point source of light, in consequence, is a series of concentric light and dark bands (coloured bands with white light), a diffraction pattern.


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Glycerol effect on Beta-galactosidase

... samplings of the flasks at standard intervals from a shaker bath. After we sorted the samples we put them into a spectrometer and read their diffraction. From the charts generated, the solution with TMG had the correct effect of producing B-galactosidase. The glucose solution expectantly ...

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by ScienceRob
Wed Apr 14, 2010 11:47 pm
 
Forum: Microbiology
Topic: Glycerol effect on Beta-galactosidase
Replies: 3
Views: 3882

Re: Difference between spectrocopy

... are measuring the light that the sample transmits (or absorbs, it's the same process but a different way of reporting the quantity). A motorized diffraction grating rotates in the light path, determining what wavelength of light is passing through the sample. With a spectrofluorometer (I have ...

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by jonmoulton
Tue Nov 17, 2009 6:38 pm
 
Forum: General Discussion
Topic: Difference between spectrocopy
Replies: 3
Views: 2874

Egg Yolk Coacervates & pH Imaging

... will make a microscope that would be comparable to what goes for almost $1000 on ebay because of phase contrast being added. I also have research diffraction grating and front surface mirror lenses to eliminate going through glass even. If it's possible to way outdo the $220 dollar 1600x without ...

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by GaryGaulin
Tue Mar 03, 2009 3:02 pm
 
Forum: General Discussion
Topic: Egg Yolk Coacervates & pH Imaging
Replies: 0
Views: 3185

Re: X Ray crystallography & transmembrane proteins

... the bilayer. You can extract structural information from these kinds of assemblies, but usually it is low resolution information derived from diffraction experiments in an electron microscope, not from higher resolution x-rays; the resolution of 2D crystal structures used to be around 20-30 ...

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by blcr11
Fri Sep 05, 2008 11:43 am
 
Forum: Molecular Biology
Topic: X Ray crystallography & transmembrane proteins
Replies: 5
Views: 3897

lipid optics

... aerated, etc.) does the solid water become opaque. So it seems that the transparent liquid to opaque solid transition in water is due to repeated diffraction and scattering, leading to reflection. The same thing has to be happening in the wax but I can't figure out molecularly how the phase would ...

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by dae
Wed Apr 09, 2008 2:51 am
 
Forum: General Discussion
Topic: lipid optics
Replies: 1
Views: 1692
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