Dictionary » D » Differential stain

Differential stain



The dye or stain that is used to differentiate one component or cellular structure from another, or to differentiate an entity from another in a specimen. It does so by colouring a portion of a specimen (e.g. of a tissue or of a cell) that remained uncoloured following the first staining using a dye of different color.


For instance, in Gram staining, two dyes are used: the Crystal Violet and Safranin. The Safranin is the counterstain used in this method. The Gram Staining is used to distinguish Gram positive bacteria from Gram negative bacteria.


Please contribute to this project, if you have more information about this term feel free to edit this page

Results from our forum

Re: Staining slides with Food dye? (Or any household item)

... for staining - I've never tried it. Either should be suitable for non-differential staining of bacteria. If you need to Gram Stain, I think you really need to use Crystal Violet counterstained with either Safranin or ...

See entire post
by billw
Fri Jan 22, 2010 12:51 am
Forum: Microbiology
Topic: Staining slides with Food dye? (Or any household item)
Replies: 7
Views: 10143

Endospore stain

Endospore stain is a differential staining procedure that allows to see both spores and vegetative cells, thus including separate -ve ...

See entire post
by Cat
Wed Feb 20, 2008 8:33 pm
Forum: Microbiology
Topic: Endospore stain
Replies: 2
Views: 22053

The Fiber Disease

... isolated easily from the vesicular lesions and can be observed on Gram stain. The initial lesion ruptures after a week and progresses to a characteristic black eschar. Differential diagnoses of the skin lesion include tularemia, plague, cutaneous diphtheria, ...

See entire post
by Skytroll
Mon Aug 28, 2006 3:17 pm
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 6245224

dna testing

... this site: Separation of ionic molecules, (principally proteins) by the differential migration through a gel according to the size and ionic charge ... Similar molecules will group on the gel. They may be visualised by staining and quantitated, in relative terms, using densitometers which continuously ...

See entire post
by biostudent84
Tue Dec 14, 2004 7:51 pm
Forum: Molecular Biology
Topic: dna testing
Replies: 4
Views: 6302

This page was last modified 08:18, 5 October 2009. This page has been accessed 34,919 times. 
What links here | Related changes | Permanent link