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Dideoxynucleotide

Dideoxynucleotide

(Science: biochemistry) a deoxynucleotide that lacks a 3' hydroxyl group, and is thus unable to form a 3'-5' phosphodiester bond necessary for chain elongation. Dideoxynucleotides are used in dna sequencing and the treatment of viral diseases.


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dideoxy chain-termination method for sequencing DNA

... enough to separate molecule that differ in only one base pair. However, what the laser detector sees at each step is not the individual tagged dideoxynucleotide, but actually the whole DNA molecule. In other words, you only see the tagged nucleotide because it is the only one tagged, but you ...

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by MrMistery
Mon Dec 15, 2008 4:56 am
 
Forum: Molecular Biology
Topic: dideoxy chain-termination method for sequencing DNA
Replies: 9
Views: 1654


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