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Detergents

Detergents

Purifying or cleansing agents, usually salts of long-chain aliphatic bases or acids, that exert cleansing (oil-dissolving) and antimicrobial effects through a surface action that depends on possessing both hydrophilic and hydrophobic properties.


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Western Blot

... that can be used is to separate it according to how big it is, otherwise known as its molecular weight. When extracts are prepared from a cell, detergents can be used to make the proteins unfold into the linear chain of amino acids. Using a detergent such as sodium dodecyl sulfate (SDS) makes ...

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by MarkHolland
Sat Mar 30, 2013 3:22 pm
 
Forum: Cell Biology
Topic: Western Blot
Replies: 6
Views: 3420

Re: Artificial phosphorylation by using different detergents

Thanks :-) And is it possible that some signaling pathways, which are investigated, can be described with mistakes due to using of different detergents :shock: ? Is there any publication about it?

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by MrsHappyy
Tue Apr 17, 2012 8:04 am
 
Forum: Cell Biology
Topic: Artificial phosphorylation by using different detergents
Replies: 2
Views: 1533

Artificial phosphorylation by using different detergents

Is it possible that using of different detergents (for isolation of lipid microdomains) has any influence on different phosphorylation of proteins in signaling pathways? Thanks for your anwers

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by MrsHappyy
Tue Apr 17, 2012 6:19 am
 
Forum: Cell Biology
Topic: Artificial phosphorylation by using different detergents
Replies: 2
Views: 1533

Re: Developing resistance to detergent

... that destroys the antibiotic. But quite a lot of chemicals are too unsubtle in their action for such mechanisms of resistance to work. I suspect detergents are among them, since they dissolve fatty materials. Such fatty materials are part of the cell membrane. Destroy the cell membrane and the ...

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by skeptic
Sun May 29, 2011 4:28 am
 
Forum: Microbiology
Topic: Developing resistance to detergent
Replies: 2
Views: 2399

Developing resistance to detergent

Firstly, detergents are not bactericid, they are supposed to remove the crude dirt. That's way bacteria don't need any resistance to soap. Contrary, if the higher concentrations were more deadly, the bacteria would be pushed even ...

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by JackBean
Tue May 24, 2011 9:33 am
 
Forum: Microbiology
Topic: Developing resistance to detergent
Replies: 2
Views: 2399
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