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Detergent

Detergent

(Science: chemistry) An agent which purifies or cleanses.

(Science: cell biology) amphipathic, surface active, molecules with polar (water soluble) and nonpolar hydrophobic) domains. They bind strongly to hydrophobic molecules or molecular domains to confer water solubility.

Examples include: sodium dodecyl sulphate, fatty acid salts, the Triton family, octyl glycoside.

Origin: L. Detergere = to cleanse


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Western Blot

... that can be used is to separate it according to how big it is, otherwise known as its molecular weight. When extracts are prepared from a cell, detergents can be used to make the proteins unfold into the linear chain of amino acids. Using a detergent such as sodium dodecyl sulfate (SDS) makes ...

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by MarkHolland
Sat Mar 30, 2013 3:22 pm
 
Forum: Cell Biology
Topic: Western Blot
Replies: 6
Views: 3273

Mechanism of Saponin in laboratory use?

... I couldn't really find anything useful. As far as I know, it's used to get stuff inside the cells, but I can't figure out whether it's a regular detergent or some kind of vesicle forming compound that 'transports' whatever it is you want, inside. Anyone has a clue?

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by nukeman
Mon Dec 03, 2012 11:57 am
 
Forum: Molecular Biology
Topic: Mechanism of Saponin in laboratory use?
Replies: 0
Views: 965

Chromatin experiment

... the following protocols modifications could remove the high molecular weight bands? 1. Increase the EDTA concentration 2. Expose the cells to the detergent for longer time 3. Increase the concentration of the nuclease 4. Decrease the concentration of the nuclease 5. Increase the incubation time ...

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by 2468
Thu Nov 08, 2012 7:48 pm
 
Forum: Molecular Biology
Topic: Chromatin experiment
Replies: 10
Views: 3197

Growth on metal pot?!

maybe some kind of mold, be sure to clean your pot and dont have any food debri left on it.Soak it in warm water with detergent, and scrub, you can use chlorine also. ;)

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by michimichi
Fri Jun 01, 2012 3:18 pm
 
Forum: Microbiology
Topic: Growth on metal pot?!
Replies: 24
Views: 9839

Removing MBP tag

... to do your cutting out of the column and add whatever to the solution to resolve interactions (8M urea, 6M Guanidium HCl, your choice of salts and detergent?) and then dialyse the hell out of if your protein is smaller than the MBP it or use a selection of Amicon filters (or something similar) ...

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by canalon
Tue Aug 16, 2011 5:52 pm
 
Forum: Molecular Biology
Topic: Removing MBP tag
Replies: 12
Views: 8693
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