Dictionary » D » Detergent



(Science: chemistry) An agent which purifies or cleanses.

(Science: cell biology) amphipathic, surface active, molecules with polar (water soluble) and nonpolar hydrophobic) domains. They bind strongly to hydrophobic molecules or molecular domains to confer water solubility.

Examples include: sodium dodecyl sulphate, fatty acid salts, the Triton family, octyl glycoside.

Origin: L. Detergere = to cleanse

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Basic Knowledge about DNA Extraction

... been proved that vortexing with phenol is often a effective method to break down protienacious cellular walls or viral capsids and the using of a detergent such as SDS is viable to remove lipid membranes. Besides, DNA associated with other cellular proteins can be degraded with the addition of ...

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by 2444256703
Thu Jan 22, 2015 8:56 am
Forum: Genetics
Topic: Basic Knowledge about DNA Extraction
Replies: 0
Views: 738

P2 Safety

... Staphylo and Streptococcus. I always disinfect workplace with 70% ethanol. I also clean the floor from time to time (not really often), with some detergent. However, I am mostly concerned about airborne contamination. 1) Is it possible to breath in some bacteria when I open the ependorf containing ...

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by SpellForce
Thu Oct 02, 2014 8:13 pm
Forum: Microbiology
Topic: P2 Safety
Replies: 1
Views: 1182

Western Blot

... that can be used is to separate it according to how big it is, otherwise known as its molecular weight. When extracts are prepared from a cell, detergents can be used to make the proteins unfold into the linear chain of amino acids. Using a detergent such as sodium dodecyl sulfate (SDS) makes ...

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by MarkHolland
Sat Mar 30, 2013 3:22 pm
Forum: Cell Biology
Topic: Western Blot
Replies: 6
Views: 4424

Mechanism of Saponin in laboratory use?

... I couldn't really find anything useful. As far as I know, it's used to get stuff inside the cells, but I can't figure out whether it's a regular detergent or some kind of vesicle forming compound that 'transports' whatever it is you want, inside. Anyone has a clue?

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by nukeman
Mon Dec 03, 2012 11:57 am
Forum: Molecular Biology
Topic: Mechanism of Saponin in laboratory use?
Replies: 0
Views: 1246

Chromatin experiment

... the following protocols modifications could remove the high molecular weight bands? 1. Increase the EDTA concentration 2. Expose the cells to the detergent for longer time 3. Increase the concentration of the nuclease 4. Decrease the concentration of the nuclease 5. Increase the incubation time ...

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by 2468
Thu Nov 08, 2012 7:48 pm
Forum: Molecular Biology
Topic: Chromatin experiment
Replies: 10
Views: 4617
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