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Gene regulation

... the level of cbbA product. Rationalize the following possible results: Case A. The cbbA product is present at a very high level. Case B. He detects approximately wild-type levels of the cbbA gene product. Case C. Drug-resistant transformants of the second plasmid are hard to get and, when ...

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by tteessffaa
Fri Nov 30, 2012 8:44 am
Forum: Molecular Biology
Topic: Gene regulation
Replies: 0
Views: 1118

Primer Extension Assay

Could somebody please explain to me how the primer extension assay detects the 5' end of the transcription start site? I found the following diagram of the assay: http://www.biochem.arizona.edu/classes/bioc568/primer_extension.htm However, that diagram is ...

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by Danja
Sat Nov 05, 2011 6:28 pm
Forum: Molecular Biology
Topic: Primer Extension Assay
Replies: 2
Views: 1864

Re: Re:

... did not want to use a dye or a chemical compound. Well, in this light phenol red might work indeed, though it is quite non-specific since it only detects the pH change. I cannot come up with any other such means right not, but I'll let you know if something occurs to me. edit: You could look for ...

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by biohazard
Tue Jun 28, 2011 6:00 am
Forum: Microbiology
Topic: Identification of bacterial growth
Replies: 6
Views: 5406

Creation of virus?

... response to different mutations as they relie on vastly different methods of detection. But since those tests are quite reliable, (the western detects some larger feature of the virus while the ELISA detects the Antibody targeted against it) while the virus is known to be mutating a lot by ...

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by canalon
Thu Mar 17, 2011 4:06 pm
Forum: Microbiology
Topic: Creation of virus?
Replies: 10
Views: 4620

spindle checkpoint - checkpoint signals

... Checkpoints are activated through the cell cycle by a series of sensors that recognize DNA damage of cellular abnormalities. If a sensor detects the presence of a defect, it triggers a response that temporarily holds further cell cycle progress so repairs can be made. This is important ...

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by Hudson
Tue Mar 15, 2011 6:33 pm
Forum: Cell Biology
Topic: spindle checkpoint - checkpoint signals
Replies: 0
Views: 2306
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