Detector

When you increased the analyte concentration, did the machine calibrate gain before the first cycle?

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care to elaborate how you solved the problem? I used to use Roche light cycler 480 and now I am using RotorQgene and for the RotorQ it optimizes the gain before the run and sometimes during the run. I also found out that the intensity of the fluorescnce depends on how many tubes are in the rotor. I ...

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Thanks! I already solved the problem.

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... no signal, the gain is set low, perhaps to avoid setting the highest possible gain when calibrating against an absent signal then blowing out the detector when a strong signal is initiated with the gain set too high. With some signal, the gain is set so that the signal falls somewhere toward the ...

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Can anyone explain what GAIN in fluorescence detector actually is? I am running real-time PCR and the signal seems too low for detection if I calibrate gain "before the 1st cycle". If I recalibrate in the middle of the run, I see signal. ...

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