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Detector

Detector

One who, or that which, detects; a detecter. A deathbed's detector of the heart. (Young) bank-note detector, a publication containing a description of genuine and counterfeit bank notes, designed to enable persons to discriminate between them. Detector lk. See lock.

Origin: L, a revealer.


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Re: what is gain in fluorescence detector

When you increased the analyte concentration, did the machine calibrate gain before the first cycle?

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by jonmoulton
Fri Oct 12, 2012 2:40 pm
 
Forum: Molecular Biology
Topic: what is gain in fluorescence detector
Replies: 6
Views: 2704

Re: what is gain in fluorescence detector

care to elaborate how you solved the problem? I used to use Roche light cycler 480 and now I am using RotorQgene and for the RotorQ it optimizes the gain before the run and sometimes during the run. I also found out that the intensity of the fluorescnce depends on how many tubes are in the rotor. I ...

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by bravebeaker
Fri Oct 12, 2012 12:44 am
 
Forum: Molecular Biology
Topic: what is gain in fluorescence detector
Replies: 6
Views: 2704

Re: what is gain in fluorescence detector

Thanks! I already solved the problem.

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by Cat
Thu Oct 11, 2012 1:44 am
 
Forum: Molecular Biology
Topic: what is gain in fluorescence detector
Replies: 6
Views: 2704

Re: what is gain in fluorescence detector

... no signal, the gain is set low, perhaps to avoid setting the highest possible gain when calibrating against an absent signal then blowing out the detector when a strong signal is initiated with the gain set too high. With some signal, the gain is set so that the signal falls somewhere toward the ...

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by jonmoulton
Tue Oct 09, 2012 10:30 pm
 
Forum: Molecular Biology
Topic: what is gain in fluorescence detector
Replies: 6
Views: 2704

what is gain in fluorescence detector

Can anyone explain what GAIN in fluorescence detector actually is? I am running real-time PCR and the signal seems too low for detection if I calibrate gain "before the 1st cycle". If I recalibrate in the middle of the run, I see signal. ...

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by Cat
Tue Oct 09, 2012 12:37 am
 
Forum: Molecular Biology
Topic: what is gain in fluorescence detector
Replies: 6
Views: 2704
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