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Detection

Detection

Act of detecting, discovery, the laying open of what was concealed or hidden or of what tends to elude observation.


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Recommendations for Bacteria Detection?

Hi all, I apologize if this is a dumb question-I'm quite new to the biology field and to be honest at this point I'm not even sure if I'm asking the right things. I am wondering what the best way to do a field test on, say, a lake or puddle, to check the number of bacteria present in the water. I'm ...

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by lodestone25
Sun Jul 27, 2014 6:20 pm
 
Forum: Microbiology
Topic: Recommendations for Bacteria Detection?
Replies: 1
Views: 353

centrifugation of hemocytes

... resuspension, since the cells are pelleted because of centrifugation, not because they grew there. What about trying some Western or other protein detection after the resuspension to see if there is anything at all? If you'll get some signal, you get the cells pelleted, they are just not viable. ...

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by JackBean
Thu Jan 30, 2014 9:34 am
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 2373

Re: centrifugation of hemocytes

... and gently take off the fresh-washing-PBS before really resuspending and i'll see... I guess the pellet can't be viewed (with eye's limit of detection..) at this amount of cells... Thank you for the comments,

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by Thom
Tue Jan 28, 2014 4:16 pm
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 2373

electrophoretic mobility shift assay (EMSA)

I am doing EMSA and after the first try having success, I could never reproduce my results: following the streptavidin-biotin-HRP detection, there are no bands on the blot paper. Compared to the first succesful try, the most striking difference I experience is that while at first, there ...

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by kk
Fri Nov 08, 2013 12:21 pm
 
Forum: Molecular Biology
Topic: electrophoretic mobility shift assay (EMSA)
Replies: 1
Views: 1149

hybridization probe

I'd say it depends solely on your possibilities with the machine (excitation and detection range), ootherwise it shouldn't matter much.

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by JackBean
Tue Sep 17, 2013 3:43 pm
 
Forum: Genetics
Topic: hybridization probe
Replies: 2
Views: 1448
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