Dictionary » D » Detection

Detection

Detection

Act of detecting, discovery, the laying open of what was concealed or hidden or of what tends to elude observation.


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centrifugation of hemocytes

... resuspension, since the cells are pelleted because of centrifugation, not because they grew there. What about trying some Western or other protein detection after the resuspension to see if there is anything at all? If you'll get some signal, you get the cells pelleted, they are just not viable. ...

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by JackBean
Thu Jan 30, 2014 9:34 am
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 654

Re: centrifugation of hemocytes

... and gently take off the fresh-washing-PBS before really resuspending and i'll see... I guess the pellet can't be viewed (with eye's limit of detection..) at this amount of cells... Thank you for the comments,

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by Thom
Tue Jan 28, 2014 4:16 pm
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 654

electrophoretic mobility shift assay (EMSA)

I am doing EMSA and after the first try having success, I could never reproduce my results: following the streptavidin-biotin-HRP detection, there are no bands on the blot paper. Compared to the first succesful try, the most striking difference I experience is that while at first, there ...

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by kk
Fri Nov 08, 2013 12:21 pm
 
Forum: Molecular Biology
Topic: electrophoretic mobility shift assay (EMSA)
Replies: 1
Views: 648

hybridization probe

I'd say it depends solely on your possibilities with the machine (excitation and detection range), ootherwise it shouldn't matter much.

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by JackBean
Tue Sep 17, 2013 3:43 pm
 
Forum: Genetics
Topic: hybridization probe
Replies: 2
Views: 1079

Hereditary Diseases - How to get rid of them?

... know much about Biology but I believe it is unlikely anything can be done once the foetus is in the womb - the only thing being possible being detection, making things like IVF the way to have contact with the Sperm & Egg before the foetus develops. Is it possible to detect these illnesses/ ...

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by DondiLion
Thu Aug 15, 2013 1:33 am
 
Forum: Genetics
Topic: Hereditary Diseases - How to get rid of them?
Replies: 2
Views: 2049
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