Derivatives

... the food differently. And depending on what the meal consists of (high protein. low carbs, high fat, even burnt meat with its cholesterol derivatives), different reactions begin different cascades thru out the body. Even the immune system gets involved. Insulin is a hormone that needs ...

See entire post

... to make a diagnostic test for a virus you need a protein extracted from a particular virus. But if there is no virus, there is no protein and its derivatives. All existing tests for HIV are not performed in this manner, they do not diagnose the virus itself, but merely trying to guess the existence ...

See entire post

... because it gives a clean reaction with free primary and secondary amine groups. DNP-amino acids are stable under acid hydrolysis and yield yellow derivatives that are readily identified. You can only identify the N-terminal amino acid with FDNB. Sequential Edman degradations and/or mass spectroscopy ...

See entire post

... expression. If you're after plasmid, I would recommend streaking first. If the frozen culture is a plasmid in an expressor line (like BL21 or its derivatives), and you're lazy like I am, you can make a fresh starter culture directly from the frozen stock without troubling yourself with re-plating ...

See entire post

... continued for another 5 min to scavenge excess FNBT. After cooling the mixture in an ice basket, the N-2′-nitro-4′-trifluoromethylphenyl (NTP) derivatives of polyamines were extracted twice with 2 ml of 2-methylbutane." There's nothing there that suggests to me that the histidine is dissolved ...

See entire post