Dictionary » D » Dephosphorylation

Dephosphorylation

Definition

noun, plural: dephosphorylations

The removal of a phosphate group from a compound


Supplement

Dephosphorylation in living cells is a common biochemical process in which a phosphate group is removed from an organic compound through hydrolysis. An example of this is when an ATP loses a phosphate group, thus changing into ADP.

Dephosphorylation can be reversed through phosphorylation in which a phosphate group is added to an organic compound (i.e. phosphate group added to ADP, becoming ATP).


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MOLECULAR CLONING

Thanks for the reply. I understand that dephosphorylation would prevents self-ligation. My problem was that whether annealed double stranded oligos need to be modified in any way before the ligation reaction. And yes, they need a 5'-phosphate ...

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by kk
Wed Jul 10, 2013 2:58 pm
 
Forum: Molecular Biology
Topic: MOLECULAR CLONING
Replies: 9
Views: 5250

MOLECULAR CLONING

The purpose of dephosphorylation is to prevent self-ligation of empty vector. So unless your insert is very long, it should not be necessary.

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by JackBean
Fri Feb 15, 2013 9:30 pm
 
Forum: Molecular Biology
Topic: MOLECULAR CLONING
Replies: 9
Views: 5250

Re: ATP ase vs. ADP ase

... a class of enzymes that catalyze the decomposition of adenosine triphosphate (ATP) into adenosine diphosphate (ADP) and a free phosphate ion. This dephosphorylation reaction releases energy, which the enzyme (in most cases) harnesses to drive other chemical reactions that would not otherwise occur. ...

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by bellyjelly
Tue Jul 12, 2011 7:20 am
 
Forum: Molecular Biology
Topic: ATP ase vs. ADP ase
Replies: 9
Views: 10917

pET28a(+) frustrations

Are you sure that the Shrimp Alkaline Phosphatase you used is really working? And how did you recover (or purify) your plasmid after dephosphorylation? Have you tried ethanol precipitation or something? I also use pET28a(+) as the expression vector but dephosphorylate it by rAPid alkaline ...

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by patlangic
Thu Apr 07, 2011 5:36 pm
 
Forum: Molecular Biology
Topic: pET28a(+) frustrations
Replies: 1
Views: 2875

Uncontrolled cell proliferation due to mutation

I would guess d as well. Question is, where should it be localised elsewhere? Maybe the mislocalisation would cause loss of dephosphorylation process. However, the kinase activity shouldn't be activated either.

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by JackBean
Thu Oct 28, 2010 2:37 pm
 
Forum: Molecular Biology
Topic: Uncontrolled cell proliferation due to mutation
Replies: 6
Views: 6742
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