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Deletion

Deletion

(Science: genetics) a chromosome abnormality in which part of a single chromosome has been lost.


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Re: Gene deletion

Thanks for your relpy! Maybe I wasn't clear enought but I want to delete genes in my drug-multiresistants strains without changing the rest of the bacterial strain... The drug resistancy is naturally found in both plasmid and chromosome. Anyway, I am curious to know how does it works with the hetero...

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by clefg
Thu Sep 05, 2013 2:42 pm
 
Forum: Molecular Biology
Topic: Gene deletion
Replies: 5
Views: 1445

Gene deletion

... to almost all antibiotics. Does anyone knows an alternative selection for my mutants (without antibiotics) or an alternative protocol for gene deletion? Thanks for your help!

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by clefg
Wed Sep 04, 2013 3:56 am
 
Forum: Molecular Biology
Topic: Gene deletion
Replies: 5
Views: 1445

Re: Markerless mutants using suicide vector

Thanks for your response, JackBean. Markerless deletion of alleles in this bacteria have been reported (a few 10's of times in the literature) although it is reported that illegitimate recombinations are more frequent in this bacteria than other ...

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by dhkwak1
Wed Dec 19, 2012 1:04 am
 
Forum: Genetics
Topic: Markerless mutants using suicide vector
Replies: 2
Views: 1989

Markerless mutants using suicide vector

Hello, I'm wanting to generate markerless deletion mutants in my bacteria (A. baumannii) and am using a suicide vector (gentamicin resistance, SacB gene). My vector contains ~1.2kb of homologous sequences that flank both sides of my gene (~2.4kb ...

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by dhkwak1
Tue Dec 18, 2012 5:17 am
 
Forum: Genetics
Topic: Markerless mutants using suicide vector
Replies: 2
Views: 1989

Gene regulation

1. A scientist wants to study the regulation of the cbbA gene (whose gene product function is unknown). He has a strain with a deletion of the chromosomal cbbA gene and containing a low copy number plasmid with a cbbA:lacZ transcriptional fusion (the strain therefore lacks a functional ...

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by tteessffaa
Fri Nov 30, 2012 8:44 am
 
Forum: Molecular Biology
Topic: Gene regulation
Replies: 0
Views: 872
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