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Deletion

Deletion

(Science: genetics) a chromosome abnormality in which part of a single chromosome has been lost.


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Re: Any ideas for vaccine development or anti-viral drugs???

... to the plasma membrane during budding. Involvement of other vps proteins in EBOV budding has not been established. Therefore, we used VP40 deletion analysis, virus-like particle-release assays, and confocal microscopy to investigate the potential role of ESCRT-1 proteins VPS4, VPS28, and ...

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by jonmoulton
Mon Sep 15, 2014 3:04 pm
 
Forum: Cell Biology
Topic: Any ideas for vaccine development or anti-viral drugs???
Replies: 2
Views: 479

Re: microsatellite instability

OK, another question: I can see how insertion/deletion can be detected by fragment analysis, but incorporation of a wrong nucleotide wouldn't cause change in the length of amplicon, so can that also be detected by fragment analysis?

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by kk
Thu Jul 10, 2014 2:45 pm
 
Forum: Molecular Biology
Topic: microsatellite instability
Replies: 3
Views: 2519

Re: Gene deletion

Thanks for your relpy! Maybe I wasn't clear enought but I want to delete genes in my drug-multiresistants strains without changing the rest of the bacterial strain... The drug resistancy is naturally found in both plasmid and chromosome. Anyway, I am curious to know how does it works with the hetero...

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by clefg
Thu Sep 05, 2013 2:42 pm
 
Forum: Molecular Biology
Topic: Gene deletion
Replies: 5
Views: 2429

Gene deletion

... to almost all antibiotics. Does anyone knows an alternative selection for my mutants (without antibiotics) or an alternative protocol for gene deletion? Thanks for your help!

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by clefg
Wed Sep 04, 2013 3:56 am
 
Forum: Molecular Biology
Topic: Gene deletion
Replies: 5
Views: 2429

Re: Markerless mutants using suicide vector

Thanks for your response, JackBean. Markerless deletion of alleles in this bacteria have been reported (a few 10's of times in the literature) although it is reported that illegitimate recombinations are more frequent in this bacteria than other ...

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by dhkwak1
Wed Dec 19, 2012 1:04 am
 
Forum: Genetics
Topic: Markerless mutants using suicide vector
Replies: 2
Views: 2750
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